Biao Ma

TL;DR: Comparisons showed that fully human antibodies from transgenic animals were not as efficiently produced as wild-type Ig, and significant improvements were obtained when the human V-region genes were linked to the endogenous CH-region, either on large constructs or by site-specific integration.

TL;DR: A novel transgenic rat line exclusively producing chimeric Abs with human idiotypes is generated, comprising fully human variable regions with subnanomolar Ag affinity and carrying extensive somatic mutations, similarly to conventional mAbs from normal rats.

TL;DR: It is suggested that high expression, class-switching and hypermutation are linked to optimal enhancer function provided by the large regulatory region at the 3' end of the IgH locus, and exclusion of Cδ and its downstream interval region may assist recombination.

TL;DR: This work presents an innovative platform for the rapid development of diverse sets of human HCAbs that have been selected in vivo and combines antibody repertoire analysis with immunization of transgenic rats, called UniRats, that produce chimeric HCABS with fully human VH domains in response to an antigen challenge.

TL;DR: The results demonstrate that OmniFlic animals produce an abundance of antigen-specific antibodies with heavy chain clonotype diversity that is similar to what has been described with unrestricted light chain use in mammals and shows that sequence-based discovery is a highly effective and efficient way to identify a large number of diverse monoclonal antibodies to a protein target of interest.