Bimbil Graham

TL;DR: It is clear that the grasp of the factors that contribute to the activity of nucleases and phosphatases has advanced considerably over the years, thanks in part to the sustained efforts of those seeking to develop complexes with improved hydrolytic activity.

TL;DR: It is reported that the substance P (SP) neurokinin 1 receptor (NK1R) signals from endosome to induce sustained excitation of spinal neurons and pain transmission and that specific antagonism of the NK1R in endosomes with membrane-anchored drug conjugates provides more effective and sustained pain relief than conventional plasma membrane–targeted antagonists.

TL;DR: An NMR approach based on lanthanide-induced pseudocontact shifts reveals a dynamic binding mode in which basic residues in the concave side of the Syt1 C2B-domain β-sandwich interact with a polyacidic region of the SNARE complex formed by syntaxin-1 and SNAP-25.

TL;DR: Three new metal-coordinating ligands, L(1), L(2), and L(3), have been prepared by appending o-, m-, and p-xylylguanidine pendants to one of the nitrogen atoms of 1,4,7-triazacyclononane (tacn) to accelerate cleavage of the P-O bonds within the model phosphodiesters

TL;DR: Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd(3+) chelates developed for rigid positioning of the metal ion are shown to deliver outstandingly accurate distance measurements in the 6 nm range.