Brandon C. Sos

TL;DR: Improved high-throughput methods for single-nucleus droplet-based sequencing and single-cell transposome hypersensitive site sequencing are reported, which revealed regulatory elements and transcription factors that underlie cell-type distinctions and mapped disease-associated risk variants to specific cellular populations, which provided insights into normal and pathogenic cellular processes in the human brain.

TL;DR: This study describes single-nucleus ATAC-seq, a combinatorial barcoding-assisted single-cell assay for transposase-accessible chromatin that is optimized for use on flash-frozen primary tissue samples used to examine gene regulation in 15,000 nuclei comprising 20 distinct cell types in the developing mouse forebrain.

TL;DR: A mechanism for the FL observed in mice with liver-specific disruption in GH signaling is provided and the development of FL depends on both GH-dependent increases in plasma FFA and increased hepatic uptake of FFA, likely mediated by increased expression of CD36.

TL;DR: An integrative genetics approach, which combines linkage analysis, expression QTL (eQTL) mapping, causality modeling, and genetic association in outbred mice, provides strong support for Wnt9a, Rasd1, or both underlying Bmd11.

TL;DR: Results reveal what they believe to be a novel mechanism whereby sex-specific GH patterns mediate sex differences in thrombosis through coordinated changes in the expression of coagulation inhibitor genes in the liver.