Showing papers by "Bruce M. Spiegelman published in 2023"
TL;DR: This paper showed that extracellular fluid (EF) from muscle and fat tissues of mice shows a different protein composition than either serum or tissues, and used this approach to identify prosaposin as a secreted product of muscle and Fat.
4 citations
TL;DR: In this paper , mass spectrometry and cryo-EM data were used to demonstrate that the extracellular heat shock protein 90α (eHsp90α) is secreted by muscle with exercise and activates integrin αVβ5.
1 citations
TL;DR: In this paper , the RNA-binding protein RBM43 was found to suppress mitochondrial and thermogenic gene expression in a PGC1α-dependent manner and its loss protected cells from cytokine-induced mitochondrial impairment.
Abstract: Adipose thermogenesis involves specialized mitochondrial function that counteracts metabolic disease through dissipation of chemical energy as heat. However, inflammation present in obese adipose tissue can impair oxidative metabolism. Here, we show that PGC1α, a key governor of mitochondrial biogenesis and thermogenesis, is negatively regulated at the level of mRNA translation by the little-known RNA-binding protein RBM43. Rbm43 is expressed selectively in white adipose depots that have low thermogenic potential, and is induced by inflammatory cytokines. RBM43 suppresses mitochondrial and thermogenic gene expression in a PGC1α-dependent manner and its loss protects cells from cytokine-induced mitochondrial impairment. In mice, adipocyte-selective Rbm43 disruption increases PGC1α translation, resulting in mitochondrial biogenesis and adipose thermogenesis. These changes are accompanied by improvements in glucose homeostasis during diet-induced obesity that are independent of body weight. The action of RBM43 suggests a translational mechanism by which inflammatory signals associated with metabolic disease dampen mitochondrial function and thermogenesis.