J
John R. Engen
Researcher at Northeastern University
Publications - 203
Citations - 13675
John R. Engen is an academic researcher from Northeastern University. The author has contributed to research in topics: Hydrogen–deuterium exchange & Mass spectrometry. The author has an hindex of 55, co-authored 186 publications receiving 11528 citations. Previous affiliations of John R. Engen include University of New Mexico & Eppley Institute for Research in Cancer and Allied Diseases.
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Journal ArticleDOI
Novel mutant-selective EGFR kinase inhibitors against EGFR T790M
Wenjun Zhou,Dalia Ercan,Liang Chen,Cai-Hong Yun,Danan Li,Marzia Capelletti,Alexis B. Cortot,Lucian R. Chirieac,Roxana E. Iacob,Robert F. Padera,John R. Engen,Kwok-Kin Wong,Michael J. Eck,Nathanael S. Gray,Pasi A. Jänne,Pasi A. Jänne +15 more
TL;DR: It is demonstrated that functional pharmacological screens against clinically important mutant kinases represent a powerful strategy to identify new classes of mutant-selective kinase inhibitors that may be clinically more effective and better tolerated than quinazoline-based inhibitors.
Journal ArticleDOI
Hydrogen exchange mass spectrometry for the analysis of protein dynamics.
Thomas E. Wales,John R. Engen +1 more
TL;DR: The general principles of the hydrogen exchange coupled to mass spectrometry method are summarized, the latest variations on the experimental protocol that probe different types of protein movements are discussed, and other recent work and improvements in the field are reviewed.
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Targeting Bcr–Abl by combining allosteric with ATP-binding-site inhibitors
Jianming Zhang,Francisco Adrian,Wolfgang Jahnke,Sandra W. Cowan-Jacob,Allen G. Li,Roxana E. Iacob,Taebo Sim,Taebo Sim,John T. Powers,John T. Powers,Christine Dierks,Fangxian Sun,Gui Rong Guo,Qiang Ding,Barun Okram,Yongmun Choi,Amy Wojciechowski,Xianming Deng,Guoxun Liu,Gabriele Fendrich,André Strauss,Navratna Vajpai,Stephan Grzesiek,Tove Tuntland,Yi Liu,Badry Bursulaya,Mohammad Azam,Mohammad Azam,Paul W. Manley,John R. Engen,George Q. Daley,George Q. Daley,Markus Warmuth,Nathanael S. Gray +33 more
TL;DR: The results show that therapeutically relevant inhibition of Bcr–Abl activity can be achieved with inhibitors that bind to the myristate-binding site and that combining allosteric and ATP-competitive inhibitors can overcome resistance to either agent alone.
Journal ArticleDOI
Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars
TL;DR: The current state of the art in analytical technologies to assess three characteristics of protein biopharmaceuticals that regulatory authorities have identified as being important in development strategies for biosimilars: post-translational modifications, three-dimensional structures and protein aggregation are discussed.
Journal ArticleDOI
Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.
Glenn R. Masson,John E. Burke,Natalie G. Ahn,Ganesh S. Anand,Christoph H. Borchers,Sébastien Brier,George M. Bou-Assaf,John R. Engen,S. Walter Englander,Johan H. Faber,Rachel Garlish,Patrick R. Griffin,Michael L. Gross,Miklos Guttman,Yoshitomo Hamuro,Albert J. R. Heck,Damian Houde,Roxana E. Iacob,Thomas J. D. Jørgensen,Igor A. Kaltashov,Judith P. Klinman,Lars Konermann,Petr Man,Leland Mayne,Bruce D. Pascal,Dana Reichmann,Mark Skehel,Joost Snijder,Timothy S. Strutzenberg,Eric S. Underbakke,Cornelia Wagner,Thomas E. Wales,Benjamin T. Walters,David D. Weis,Derek J. Wilson,Patrick L. Wintrode,Zhongqi Zhang,Jie Zheng,David C. Schriemer,Kasper D. Rand +39 more
TL;DR: Recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017) are provided, meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.