T
Thomas E. Wales
Researcher at Northeastern University
Publications - 83
Citations - 4318
Thomas E. Wales is an academic researcher from Northeastern University. The author has contributed to research in topics: SH3 domain & Mass spectrometry. The author has an hindex of 28, co-authored 74 publications receiving 3318 citations. Previous affiliations of Thomas E. Wales include Duke University & University of New Mexico.
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Journal ArticleDOI
Hydrogen exchange mass spectrometry for the analysis of protein dynamics.
Thomas E. Wales,John R. Engen +1 more
TL;DR: The general principles of the hydrogen exchange coupled to mass spectrometry method are summarized, the latest variations on the experimental protocol that probe different types of protein movements are discussed, and other recent work and improvements in the field are reviewed.
Journal ArticleDOI
Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.
Glenn R. Masson,John E. Burke,Natalie G. Ahn,Ganesh S. Anand,Christoph H. Borchers,Sébastien Brier,George M. Bou-Assaf,John R. Engen,S. Walter Englander,Johan H. Faber,Rachel Garlish,Patrick R. Griffin,Michael L. Gross,Miklos Guttman,Yoshitomo Hamuro,Albert J. R. Heck,Damian Houde,Roxana E. Iacob,Thomas J. D. Jørgensen,Igor A. Kaltashov,Judith P. Klinman,Lars Konermann,Petr Man,Leland Mayne,Bruce D. Pascal,Dana Reichmann,Mark Skehel,Joost Snijder,Timothy S. Strutzenberg,Eric S. Underbakke,Cornelia Wagner,Thomas E. Wales,Benjamin T. Walters,David D. Weis,Derek J. Wilson,Patrick L. Wintrode,Zhongqi Zhang,Jie Zheng,David C. Schriemer,Kasper D. Rand +39 more
TL;DR: Recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017) are provided, meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.
Journal ArticleDOI
High-speed and high-resolution UPLC separation at zero degrees Celsius.
TL;DR: Construction and validation of a custom UPLC system for HXMS based on the Waters nanoACQUITY platform and contains a Peltier-cooled module that houses the injection and switching valves, online pepsin digestion column, and C-18 analytical separation column are presented.
Journal ArticleDOI
Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis.
TL;DR: A data interpretation method involving peak width analysis that makes characterization of EX1 kinetics simple and rapid is described and is robust, capable of automation, and provides quick interpretation of the key information contained in EX1 kinetic events.
ComponentDOI
Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding
Edward C. Twomey,Zhejian Ji,Thomas E. Wales,Nicholas O. Bodnar,Scott B. Ficarro,Scott B. Ficarro,Jarrod A. Marto,Jarrod A. Marto,John R. Engen,Tom A. Rapoport +9 more
TL;DR: Cryo-EM structures of the Cdc48 ATPase in complex with Ufd1/Npl4 and poly-ubiquitinated substrate show that the CDC48 complex initiates substrate processing by unfolding a ubiquitin molecule.