Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction

Although conventional chemotherapy has been successful to some extent, the main drawbacks of chemotherapy are its poor bioavailability, high-dose requirements, adverse side effects, low therapeutic indices, development of multiple drug resistance, and non-specific targeting. The main aim in the development of drug delivery vehicles is to successfully address these delivery-related problems and carry drugs to the desired sites of therapeutic action while reducing adverse side effects. In this review, we will discuss the different types of materials used as delivery vehicles for chemotherapeutic agents and their structural characteristics that improve the therapeutic efficacy of their drugs and will describe recent scientific advances in the area of chemotherapy, emphasizing challenges in cancer treatments.

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Controlled drug delivery vehicles for cancer treatment and their performance.

Textbook of histology

Natural and synthetic polymers for wounds and burns dressing

Melatonin, the major hormone produced by the pineal gland, displays characteristic daily and seasonal patterns of secretion. These robust and predictable rhythms in circulating melatonin are strong synchronizers for the expression of numerous physiological processes in photoperiodic species. In mammals, the nighttime production of melatonin is mainly driven by the circadian clock, situated in the suprachiasmatic nucleus of the hypothalamus, which controls the release of norepinephrine from the dense pineal sympathetic afferents. The pivotal role of norepinephrine in the nocturnal stimulation of melatonin synthesis has been extensively dissected at the cellular and molecular levels. Besides the noradrenergic input, the presence of numerous other transmitters originating from various sources has been reported in the pineal gland. Many of these are neuropeptides and appear to contribute to the regulation of melatonin synthesis by modulating the effects of norepinephrine on pineal biochemistry. The aim of this review is firstly to update our knowledge of the cellular and molecular events underlying the noradrenergic control of melatonin synthesis; and secondly to gather together early and recent data on the effects of the nonadrenergic transmitters on modulation of melatonin synthesis. This information reveals the variety of inputs that can be integrated by the pineal gland; what elements are crucial to deliver the very precise timing information to the organism. This also clarifies the role of these various inputs in the seasonal variation of melatonin synthesis and their subsequent physiological function.

Generation of the Melatonin Endocrine Message in Mammals: A Review of the Complex Regulation of Melatonin Synthesis by Norepinephrine, Peptides, and Other Pineal Transmitters

This study was carried out to evaluate the effects of melatonin entrapped PLA nanoparticles (Mel-NPs) and pure Mel on impact of reactive oxygen species (ROS), blastogenic responses (%SR) in terms of proliferation of splenocytes. ROS have been proposed to play an important role in balancing the pro- and antioxidant homeostasis during different physiological condition of organism. Melatonin has been suggested as an effective free radical scavenger that may have an important role during this process. Mel-NPs were prepared by using emulsification (o/w) nanoprecipitation method and their physicochemical characterizations were studied for evaluation of particle size and encapsulation efficiency by using SEM, TEM, particle size analyzer, DSC and FTIR. The TEM micrograph showed excellent findings regarding size, shape, encapsulation efficiency and difference between pure PLA nanoparticles and Mel-NPs. The SEM micrographs of Mel-NPs showed spherical shape, smooth surfaces and the homogeneous particle size distribution. In TEM micrograph, the average diameter of Mel-NPs was found to be 36 ± 8 nm with ∼78% (w/w) of entrapment efficiency. The size of pure PLA nanoparticles was found to be larger as compared to Mel-NPs which confirm that the drug supports the reduction of the size of Mel-NPs. In in vitro study, we observed that Mel-NPs significantly reduce the level of ROS in splenocytes and increased the level %SR at 96 h of splenocytes culture as compared to the culture with pure Mel. Thus our results propose high clinical value for improvement of depressed immunity by Mel-NPs. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3034–3044, 2015.

Synthesis and in vitro evaluation of melatonin entrapped PLA nanoparticles: an oxidative stress and T-cell response using golden hamster.

Todate, report about the role of pineal gland in maintaining the normal physiology of gestation is scanty. Present study is the first of its kind giving a detail profile of organ weights and plasma concentration of melatonin, estradiol and progesterone to suggest a possible role of pineal gland in maintaining normal physiology during gestation and post-parturition periods of female Indian palm squirrel F. pennanti. Inspite of, inverse pineal-gonadal/melatonin-steroids interrelationship in adult (non-pregnant) females, the present results study suggest a direct relationship of pineal gland activity with ovarian steroids especially during the gestation period. The inverse relationship of melatonin and ovarian steroids is again established after parturition and maintained throughout the life. Thus the pineal gland (activity as judged by its weight, biochemical contents i.e. protein and cholesterol and plasma melatonin level) maintained ovarian/uterine physiology and regulated plasma concentrations of estradiol and progesterone during gestation and post-parturition periods. It is suggested that the pineal gland and its hormone melatonin play an important role to maintain the normal physiology of gestation and the post-partum recovery in Indian palm squirrel F. pennanti.

http://nopr.niscair.res.in/bitstream/123456789/24101/1/IJEB%2038%2810%29%20974-981.pdf

Profile of organ weights and plasma concentrations of melatonin, estradiol and progesterone during gestation and post-parturition periods in female Indian palm squirrel Funambulus pennanti.

To explain the complex mechanism of environmental influence along with internal hormonal (factors) milieu on daily variations in the circulating levels of melatonin, testosterone, thyroxine and corticosterone were analyzed with the help of inferential statistics (Cosinor rhythmometry) in a seasonally breeding tropical rodent, F. pennanti during the reproductively active (RAP) and inactive phases (RIP). Plasma melatonin, thyroxine and corticosterone levels exhibited a significant circadian oscillation during both the active and inactive phases of the annual reproductive cycle. Melatonin showed higher amplitude during RIP in the circulating plasma. Testosterone presented a peak level during evening hours (16:00 – 18:00 h) during RAP only. The phase of thyroxine was noted ∼09:76 h and ∼10.35 h during active and inactive phases, respectively. Corticosterone showed a peak level at ∼12.00 h during both phases of the reproductive cycle. Further, in this tropical rodent, the minimum difference in photope...

Reproductive phase dependent circadian variations of plasma melatonin, testosterone, thyroxine and corticosterone in Indian palm squirrel, Funambulus pennanti

Daily variation of plasma melatonin affects daily activity pattern of many birds, but daily immunoprotective activity of melatonin in any seasonally breeding avian species is lacking. We report the influence of endogenous melatonin and testosterone on the daily variation in immunity of the Indian tropical bird Perdicula asiatica during reproductively active (RAP) and inactive (RIP) periods when the level of melatonin was high and in the former case. Daily variation in levels of melatonin, testosterone and immune parameters was noted during RAP and RIP. Maximum immune activity was noted at 2:00 hrs during RAP and at 14:00 hrs during RIP. During RAP, high testosterone in the circulation suppressed melatonin levels and immune parameters. A high basal level of melatonin during RIP was responsible for the suppression of testosterone resulting in high immune activity. Therefore, along with testosterone, melatonin acts like a major temporal synchronizer to maintain not only the reproductive rhythm but a...

Chandana Haldar

Papers

Synthesis and in vitro evaluation of melatonin entrapped PLA nanoparticles: an oxidative stress and T-cell response using golden hamster.

Profile of organ weights and plasma concentrations of melatonin, estradiol and progesterone during gestation and post-parturition periods in female Indian palm squirrel Funambulus pennanti.

Reproductive phase dependent circadian variations of plasma melatonin, testosterone, thyroxine and corticosterone in Indian palm squirrel, Funambulus pennanti

Biological significance of daily variation in immunity of Perdicula asiatica: role of melatonin and testosterone

Role of melatonin and HSF-1\HSP-70 in modulating cold stress-induced immunosuppression in a tropical rodent- Funambulus pennanti.