Christian Henn

TL;DR: Topographs of two-dimensional porin OmpF crystals reconstituted in the presence of lipids were recorded in solution by atomic force microscopy (AFM), demonstrating the potential of AFM to monitor conformational changes with high resolution.

TL;DR: Three-dimensional reconstruction from sodium silicotungstate-stained specimens revealed the AUM particles to be built of six "V-shaped" subunits anchored upright in the membrane, a first glimpse of the molecular architecture of the 16 nm particles constituting the plaques.

TL;DR: This review critically evaluates the potential and the limitations in obtaining molecular resolution, particularly with negatively stained specimens, and puts these into perspective with cryomicroscopy of unstained frozen-hydrated and sugar-embedded preparations.

TL;DR: In this article, real-time texture mapping capabilities of modern graphics workstations are explored with respect to their applications in a variety of relevant scenarios in interactive molecular modeling techniques, such as display and filter multichannel information of structural properties on molecular surfaces, improve the quality and accuracy of highly complex isodensity contours, increase the rendering speed of space-filling atomic representations by two orders of magnitude and apply volume-rendering techniques to large, three-dimensional density distributions in real time.

TL;DR: Evidence supporting this model results from quasielastic light scattering analyses and electron microscopy of different lipid-detergent systems and reconstitution experiments with Escherichia coli porin OmpF, Phormidium laminosum photosystem I reaction centers, and integral membrane proteins of mammalian lens fiber cells.