Showing papers by "Chung-Ming Hsieh published in 1998"
TL;DR: Using a DNA probe from the zinc finger region of EKLF, this article cloned a cDNA encoding a member of this family from a human vascular endothelial cell cDNA library.
188 citations
TL;DR: Using a recently developed neural crest cell to smooth muscle cell in vitro differentiation system, it is found that ACLP mRNA and protein were not expressed in neural crest cells but were up-regulated dramatically with the differentiation of these cells, indicating that ACLp may play a role in differentiated vascular smooth muscle cells.
89 citations
TL;DR: This Monc-1 to SMC model should facilitate the identification of nodal regulators of smooth muscle development and differentiation and identify new DNA-protein complexes in nuclear extracts prepared from differentiated MonC-1 cells.
63 citations
TL;DR: An important role for this gene family in the development of smooth and striated muscle is suggested and its expression after gastrulation in developing mice is analyzed and compared with that of the other 2 members of the CRP subclass.
Abstract: Proteins of the LIM family are critical regulators of development and differentiation in various cell types. We have described the cloning of cysteine-rich protein 2/smooth muscle LIM protein (CRP2/SmLIM), a LIM-only protein expressed in differentiated vascular smooth muscle cells. As a first step toward understanding the potential functions of CRP2/SmLIM, we analyzed its expression after gastrulation in developing mice and compared the expression of CRP2/SmLIM with that of the other 2 members of the CRP subclass, CRP1 and CRP3/MLP. In situ hybridization in whole-mount and sectioned embryos showed that CRP2/SmLIM was expressed in the sinus venosus and the 2 cardiac chambers at embryonic day 9. Vascular expression of CRP2/SmLIM was first seen at embryonic day 10. At subsequent time points, CRP2/SmLIM expression decreased in the heart but remained high in the vasculature. CRP1 was expressed both in vascular and nonvascular tissues containing smooth muscle cells, whereas CRP3/MLP was expressed only in tissues containing striated muscle. These patterns of expression were maintained in the adult animal and suggest an important role for this gene family in the development of smooth and striated muscle.
62 citations
TL;DR: In vivo, the 5-kilobase fragment directed high level expression of the lacZ reporter gene preferentially in the VSMC of transgenic mice, indicating the presence of VSMC-specific element(s) in this fragment.
45 citations
Patent•
06 Feb 1998
TL;DR: Aortic-preferentiallyexpressed gene-1 (APEG-1) and striated muscle preferentially expressed (SPEG) polypeptide, DNA sequences encoding and controlling the transcription of the APEG1/SPEG encoding gene, methods of diagnosing vascular injury, and methods of conferring smooth muscle-cell specific expression as discussed by the authors.
Abstract: Aortic-preferentially-expressed gene-1 (APEG-1) and striated muscle preferentially expressed (SPEG) polypeptide, DNA sequences encoding and controlling the transcription of the APEG-1/SPEG encoding gene, methods of diagnosing vascular injury, methods of conferring smooth muscle-cell specific expression, and methods of inhibiting vascular smooth muscle cell proliferation by increasing the level of APEG-1 at the site of vascular injury.
8 citations