https://is.muni.cz/el/1431/podzim2006/C7780/um/Read/2711172/SAM_str_grwth_ProgSurfSci00_151.pdf

Structure and growth of self-assembling monolayers

This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific adsorption of proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N-acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the adsorption of proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the adsorption of proteins. In the group of surfaces examined, those that resisted the adsorption of proteins had the following properties:  they were ...

A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein

The recruitment model for gene activation stipulates that an activator works by bringing the transcriptional machinery to the DNA. Recent experiments in bacteria and yeast indicate that many genes can be activated by this mechanism. These findings have implications for our understanding of the nature of activating regions and their targets, and for the role of histones in gene regulation.

Transcriptional activation by recruitment

The role of general initiation factors in transcription by RNA polymerase II.

/pdf/the-general-transcription-factors-of-rna-polymerase-ii-1l1zp14fl7.pdf

The general transcription factors of RNA polymerase II.

This paper reports the generation of a self-assembled monolayer (SAM) that selectively binds proteins whose primary sequence terminates with a His-tag:  a stretch of six histidines commonly incorporated in recombinant proteins to simplify purification. The SAM was prepared by the adsorption onto a gold surface of a mixture of two alkanethiols:  one thiol that terminated with a nitrilotriacetic acid (NTA) group, a group that forms a tetravalent chelate with Ni(II), and a second thiol that terminated with a tri(ethylene glycol) group, a group that resists protein adsorption. His-tagged proteins bound to the SAM by interaction of the histidines with the two vacant sites on Ni(II) ions chelated to the surface NTA groups. Studies with model proteins showed the binding was specific for His-tagged proteins and required the presence of Ni(II) on the surface. Immobilized His-tagged proteins were kinetically stable in buffered saline at pH 7.2 but could be desorbed by treatment with 200 mM imidazole. Surface plasmo...

A self-assembled monolayer for the binding and study of histidine-tagged proteins by surface plasmon resonance.

An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.

Molecular recognition at surfaces derivatized with self-assembled monolayers

https://www.cell.com/cell/pdf/0092-8674(95)90389-5.pdf

Contact with a component of the polymerase II holoenzyme suffices for gene activation.

A technique for immobilizing biological molecules, in particular nucleic acid strands, is described. Biological molecules immobilized at surfaces can be used in electron-transfer detection techniques in which a binding partner of a biological molecule is brought into proximity of the surface-immobilized biological molecule, an electrical potential created between the two biologically-binding species, and electron transfer through the species determined. Another technique involves immobilizing a biological molecule such as a protein, DNA, etc., at a surface via a self-assembled monolayer, affecting the biological molecule via, for example, biological binding, inducing a change in conformation via a prion, etc., and detecting an electronic property change in the molecule via a change in impedance associated with an electronic circuit addressed by the biological molecule. This technique facilitates combinatorial array detection articles.

Cynthia Bamdad

Papers

A self-assembled monolayer for the binding and study of histidine-tagged proteins by surface plasmon resonance.

Molecular recognition at surfaces derivatized with self-assembled monolayers

Contact with a component of the polymerase II holoenzyme suffices for gene activation.

Electronic-property probing of biological molecules at surfaces

A DNA self-assembled monolayer for the specific attachment of unmodified double- or single-stranded DNA.