Joseph Pearlberg

TL;DR: OP (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which is more effective than the previously published modular assembly method and provides an "open-source" method for rapidly engineering highly active zinc-finger arrays.

TL;DR: ErbB-3 is used to couple EGFR to the PI3K/Akt pathway in gefitinib-sensitive NSCLC cell lines harboring WT and mutant EGFRs, and dissociates this complex, thereby linking EGFR inhibition to decreased Akt activity.

TL;DR: Gefitinib treatment decreased EGFR, ERK1/2, and Akt phosphorylation in EGFR mutant cell lines whereas cetuximab had relatively little effect, and EGFR mutations in NSCLC cells are associated with sensitivity to gefit inib but not to cetUXimab.

TL;DR: It is proposed that a single activator-holoenzyme contact can trigger gene activation simply by recruiting the latter to DNA.

TL;DR: Using these methods, zebrafish founders carrying targeted mutations can be identified within 4 months, and the vectors and methods are compatible with previously described Zinc-Finger Consortium reagents for constructing engineered zinc-finger arrays.