Dag Scherfeld

TL;DR: Cholesterol was shown to promote lipid segregation in dioleoyl-phosphatidylcholine-enriched, liquid-disordered, and sphingomyelin-en enriched, liquid -ordered phases and the lipid mobility in sphingomeelin- enriched regions significantly increased by increasing the cholesterol concentration, pinpoint the key role, played by cholesterol in tuning lipid dynamics in membranes.

TL;DR: Fluorescence correlation spectroscopy promises to be a valuable tool to elucidate lipid raft associations in native cells and to gain deeper insight into the correspondence between model and natural membranes.

TL;DR: This work investigates the effect of cholesterol on mixtures of dioleoyl-phosphatidylcholine (DOPC) and dipalmitoyl (DSPC), and examines the assignment of distinct lipid phases and the molecular mobility in the membrane bilayer, as well as investigating a weaker interaction of cholesterol with saturated phosphatidycholines than with sphingomyelin.

TL;DR: Studies of combined confocal fluorescence microscopy and fluorescence correlation spectroscopy on lipid dynamics and organization in rafts assembled in GUVs prepared from various lipid mixtures which are relevant to the problem of raft formation are reviewed.

TL;DR: The ability of membrane components to arrange themselves heterogeneously within the bilayer induces the formation of microdomains and lipid dynamics in distinct phases is very low and astonishingly similar, thereby suggesting the poor ability of cholesterol in sorting short-chain saturated glycero-phospholipids and sphingomyelin.