Dana J. Huebert

TL;DR: It is proposed that bivalent domains silence developmental genes in ES cells while keeping them poised for activation, highlighting the importance of DNA sequence in defining the initial epigenetic landscape and suggesting a novel chromatin-based mechanism for maintaining pluripotency.

TL;DR: Methylation patterns at orthologous loci are strongly conserved between human and mouse even though many methylated sites do not show sequence conservation notably higher than background, which suggests that the DNA elements that direct the methylation represent only a small fraction of the region or lie at some distance from the site.

TL;DR: ChIP-on-chip methods for analyzing histone modification patterns at genome-scale in mammalian cells have been proposed in this paper, which combines the specificity of chromatin immunoprecipitation with the unbiased, high-throughput capabilities of microarrays.

TL;DR: High-throughput microarray and sequencing technologies are being combined with biochemical and immunological enrichment methods to obtain genome-scale views of chromatin in a variety of organisms, which pinpoint novel, genomic elements and expansive chromatin domains, and offer insight into the functions of histone modifications.