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Daniel Knobeloch
Researcher at Charité
Publications - 12
Citations - 619
Daniel Knobeloch is an academic researcher from Charité. The author has contributed to research in topics: Bioreactor & Hepatocyte. The author has an hindex of 8, co-authored 12 publications receiving 573 citations.
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Journal ArticleDOI
HepaRG Human Hepatic Cell Line Utility as a Surrogate for Primary Human Hepatocytes in Drug Metabolism Assessment in Vitro
Marc Lübberstedt,Ursula Müller-Vieira,Manuela Mayer,Klaus M. Biemel,Fanny Knöspel,Daniel Knobeloch,Andreas K. Nussler,Jörg C. Gerlach,Jörg C. Gerlach,Katrin Zeilinger +9 more
TL;DR: The results from this study indicate that HepaRG cells could provide a suitable alternative to pHH in pharmaceutical research and development for metabolism studies such as CYP induction or sub-chronic to chronic hepatotoxicity studies.
Journal ArticleDOI
Scaling down of a clinical three-dimensional perfusion multicompartment hollow fiber liver bioreactor developed for extracorporeal liver support to an analytical scale device useful for hepatic pharmacological in vitro studies.
Katrin Zeilinger,Thomas Schreiter,Malin Darnell,Therese Söderdahl,Marc Lübberstedt,Birgitta Dillner,Daniel Knobeloch,Andreas K. Nussler,Jörg C. Gerlach,Tommy B. Andersson +9 more
TL;DR: The down-scaled multicompartment hollow fiber technology allows stable maintenance of primary human liver cells and provides an innovative tool for pharmacological and kinetic studies of hepatic functions with small cell numbers.
Journal ArticleDOI
Analysis of drug metabolism activities in a miniaturized liver cell bioreactor for use in pharmacological studies
Stefan A. Hoffmann,Ursula Müller-Vieira,Klaus M. Biemel,Daniel Knobeloch,Sandra Heydel,Marc Lübberstedt,Andreas K. Nussler,Tommy B. Andersson,Jörg C. Gerlach,Katrin Zeilinger +9 more
TL;DR: Diclofenac metabolism, CYP activities and gene expression levels were considerably more stable inBioreactor cultures, making the novel bioreactor a useful tool for pharmacological or toxicological investigations requiring a highly physiological in vitro representation of the liver.
Book ChapterDOI
Human Hepatocytes: Isolation, Culture, and Quality Procedures
Daniel Knobeloch,Sabrina Ehnert,Lilianna Schyschka,Peter Büchler,Michael H. Schoenberg,Jörg Kleeff,Wolfgang E. Thasler,Natascha C. Nüssler,Patricio Godoy,Jan G. Hengstler,Andreas K. Nussler +10 more
TL;DR: The necessary network in the clinic and quality controls to maintain human liver cells in culture and the effect of 3D extracellular matrix in cultured cells which results in preservation of hepatocyte epithelial polarity in the form of bile canaliculi and repression of epithelial to mesenchymal transitions occurring in 2D cultures are discussed.
Journal ArticleDOI
Evaluation and optimization of hepatocyte culture media factors by design of experiments (DoE) methodology
Jia Dong,Carl-Fredrik Mandenius,Marc Lübberstedt,Thomas Urbaniak,Andreas K. Nussler,Daniel Knobeloch,Jörg C. Gerlach,Jörg C. Gerlach,Katrin Zeilinger +8 more
TL;DR: Optimization of cell culture media based on statistical experimental design methodology is a widely used approach for improving cultivation conditions and it was possible to conclude that hepatocyte growth factor, epidermal growth factors, and oncostatin M had decisive effects on the metabolic functions of primary human hepatocytes.