Publisher Summary This chapter discusses the sequencing end-labeled DNA with base-specific chemical cleavages. In the chemical DNA sequencing method, one end-labels the DNA, partially cleaves it at each of the four bases in four reactions, orders the products by size on a slab gel, and then reads the sequence from an autoradiogram by noting which base-specific agent cleaved at each successive nucleotide along the strand. This technique sequences the DNA made in and purified from cells. No enzymatic copying in vitro is required, and either single- or double-stranded DNA can be sequenced. Most chemical schemes that cleave at one or two of the four bases involve three consecutive steps: modification of a base, removal of the modified base from its sugar, and DNA strand scission at that sugar. Base-specific chemical cleavage is only one step in sequencing DNA. The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end-labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions. The chapter also discusses the electrophoresis of the chemical cleavage products on long-distance sequencing gels and a guide for troubleshooting problems in sequencing patterns.

Sequencing end-labeled DNA with base-specific chemical cleavages.

https://www.cell.com/cell/pdf/0092-8674(93)90530-4.pdf

Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans

3.7. Palladium Nanoparticles as Catalysts 888 3.8. Other Transition-Metal Complexes 888 3.9. Transition-Metal-Free Reactions 889 4. Applications 889 4.1. Alkynylation of Arenes 889 4.2. Alkynylation of Heterocycles 891 4.3. Synthesis of Enynes and Enediynes 894 4.4. Synthesis of Ynones 896 4.5. Synthesis of Carbocyclic Systems 897 4.6. Synthesis of Heterocyclic Systems 898 4.7. Synthesis of Natural Products 903 4.8. Synthesis of Electronic and Electrooptical Molecules 906

https://www.chem.ccu.edu.tw/~joyce/referance/ericyang/Chem.%20Rev/Chem.%20Rev.%202007,%20107,%20874-922.pdf

The Sonogashira Reaction: A Booming Methodology in Synthetic Organic Chemistry†

Antibodies, the most popular class of molecules providing molecular recognition needs for a wide range of applications, have been around for more than three decades. As a result, antibodies have made substantial contributions toward the advancement of diagnostic assays and have become indispensable in most diagnostic tests that are used routinely in clinics today. The development of the systematic evolution of ligands by exponential enrichment (SELEX) process, however, made possible the isolation of oligonucleotide sequences with the capacity to recognize virtually any class of target molecules with high affinity and specificity. These oligonucleotide sequences, referred to as "aptamers", are beginning to emerge as a class of molecules that rival antibodies in both therapeutic and diagnostic applications. Aptamers are different from antibodies, yet they mimic properties of antibodies in a variety of diagnostic formats. The demand for diagnostic assays to assist in the management of existing and emerging diseases is increasing, and aptamers could potentially fulfill molecular recognition needs in those assays. Compared with the bellwether antibody technology, aptamer research is still in its infancy, but it is progressing at a fast pace. The potential of aptamers may be realized in the near future in the form of aptamer-based diagnostic products in the market. In such products, aptamers may play a key role either in conjunction with, or in place of, antibodies. It is also likely that existing diagnostic formats may change according to the need to better harness the unique properties of aptamers.

Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics

Publisher Summary This chapter reviews the current state of the knowledge of transcription and translation in Caenorhabditis elegans. Transcription in eukaryotes is accomplished by three RNA polymerases, each transcribing a particular type of RNA; RNA polymerase I transcribes ribosomal RNA, RNA polymerase II transcribes mRNAs and snRNAs, and RNA polymerase III transcribes tRNAs and other low-molecular-weight RNAs. The chapter focuses on RNA polymerase II transcription. The C. elegans gene for the large subunit of RNA polymerase II has been cloned, and has shown to encode a protein that resembles its vertebrate counterparts. For most polymerase II transcribed genes in C. elegans, a sequence fitting the consensus TATA element about 30 bp upstream of the transcriptional start site is found. Recently, a cDNA encoding the TATA box binding protein, or TATA binding protein (TBP) subunit of transcription factor II D (TFIID), has been identified in the nematode. This protein shows extended regions of sequence similarity to vertebrate TBP, binds to a TATA box sequence and can substitute for vertebrate TFIID basal activity in in vitro extracts. These results illustrate a high degree of evolutionary conservation in the basic transcription machinery.

Transcription and translation.

Anti-malarial activity of N6-modified purine analogues

A number of new acyl derivatives of cytosine and 3-methylcytosines are described. Acylation normally yields N 4 - monoacyl compounds which, in hot 80% acetic acid, yield large amounts of uracil derivatives. Exceptionally, benzoyl chloride can also yield a 1 : N 4 - dibenzoate from 3-methylcytosine and a tribenzoate from cytosine. Similarly, benzoylation of cytidine yields, according to conditions, either a tetrabenzoyl or pentabenzoyl derivative.

461. The structures of some acylcytosines

Nucleotides. Part XXIV. The action of some nucleases on simple esters of monoribonucleotides

The M7.9 Wenchuan earthquake on May 12th, 2008 was the most destructive in China since 1976. The event caused huge damage and loss of life and exposed weaknesses both in the formulation and implementation of the regulations governing building in the affected provinces. Following the earthquake a massive relief and recovery operation was mounted by the Chinese government. The authors took part in field studies in the affected area which took place 5 and 11 months after the event, at which time recovery operations were well-advanced. The aims of the study were to assess the effects caused by the earthquake to the built environment and society, to collect information on the ongoing recovery efforts and future plans, and to demonstrate the use of tools that allow the collection of spatially referenced damage and recovery data. Based on available satellite imagery supplemented by ground observation, geodatabases were constructed containing information on damage and recovery in several parts of the affected area. The paper gives an overview of the recovery process, describes the methods used to construct these geodatabases, and offers some analysis of the data obtained. It is argued that such databases have great potential for the management of post-disaster recovery and for creating a permanent record of the recovery process.

The use of remotely sensed data and ground survey tools to assess damage and monitor early recovery following the 12.5.2008 Wenchuan earthquake in China

Daniel M. Brown

Papers

Anti-malarial activity of N6-modified purine analogues

461. The structures of some acylcytosines

Nucleotides. Part XXIV. The action of some nucleases on simple esters of monoribonucleotides

The use of remotely sensed data and ground survey tools to assess damage and monitor early recovery following the 12.5.2008 Wenchuan earthquake in China

A synthesis of β-2′-deoxyshowdomycin