Daniel Monreal

TL;DR: Rough mutants obtained by molecular biology methods on the knowledge of the genetics and structure of Brucella lipopolysaccharide may offer alternatives, and analyses in mice suggest that mutations affecting only the O-polysaccharides result in better vaccines than those affecting both core and O- polysaccharid.

TL;DR: Although no serological test and antigen combination fully resolved the diagnosis of bovine brucellosis in the presence of FPSR, some are simple and practical alternatives to the brucellin skin test currently recommended for differential diagnosis.

TL;DR: Results suggest that rough Brucella vaccines should carry a complete core for maximal effectiveness in order to be effective against B. abortus outer membrane topology.

TL;DR: A comparative analysis between biological activity and the calculated peptide parameters revealed that the decisive factor for a broad spectrum activity is not the peptide overall hydrophobicity or amphipathicity, but the possession of a minimal positive net charge plus a highly amphipATHic anchor point of only seven amino acid residues (two helical turns).

TL;DR: These findings correlate with the in vitro tests on methicillin resistant S. aureus, E. coli, P. aeruginosa and human red blood cells, showing increased biological activity of C12LF11 towards these test organisms, providing evidence that both electrostatic and hydrophobic interactions are crucial for biologicalActivity of antimicrobial peptides.