R
Robert H. Singer
Researcher at Albert Einstein College of Medicine
Publications - 416
Citations - 45303
Robert H. Singer is an academic researcher from Albert Einstein College of Medicine. The author has contributed to research in topics: RNA & Messenger RNA. The author has an hindex of 113, co-authored 391 publications receiving 41493 citations. Previous affiliations of Robert H. Singer include Research Institute of Molecular Pathology & Stony Brook University.
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Journal ArticleDOI
Localization of ASH1 mRNA Particles in Living Yeast
Edouard Bertrand,Pascal Chartrand,Matthias Schaefer,Shailesh M. Shenoy,Robert H. Singer,Roy M. Long +5 more
TL;DR: The ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization, and the SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation.
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Visualization of Single RNA Transcripts in Situ
Andrea M. Femino,Fredric S. Fay,Fredric S. Fay,Kevin E. Fogarty,Kevin E. Fogarty,Robert H. Singer,Robert H. Singer +6 more
TL;DR: This approach extends the power of FISH to yield quantitative molecular information on a single cell by positioning probes along the transcription unit to determine the rates of transcription initiation and termination and messenger RNA processing.
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A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
Journal ArticleDOI
Single-RNA counting reveals alternative modes of gene expression in yeast.
TL;DR: Combining single-transcript measurements with computational modeling indicates that low expression variation is achieved by transcribing genes using single transcription-initiation events that are clearly separated in time, rather than by transcriptional bursts.
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Transcriptional pulsing of a developmental gene.
TL;DR: This study directly visualized transcription of an endogenous developmental gene and found discrete "pulses" of gene activity that turn on and off at irregular intervals, which represents the first direct visualization of transcriptional pulsing in eukaryotes.