D
Darrell P. Chandler
Researcher at Argonne National Laboratory
Publications - 73
Citations - 3412
Darrell P. Chandler is an academic researcher from Argonne National Laboratory. The author has contributed to research in topics: Nucleic acid & Gene. The author has an hindex of 31, co-authored 73 publications receiving 3331 citations. Previous affiliations of Darrell P. Chandler include Pacific Northwest National Laboratory & Battelle Memorial Institute.
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Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays
TL;DR: The development and validation of a simple microarray method for the direct detection of intact 16S rRNA from unpurified soil extracts suggest that it is now possible to apply microarray technology to the direct Detection of microorganisms in environmental samples, without using PCR.
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Effect of PCR template concentration on the composition and distribution of total community 16S rDNA clone libraries
TL;DR: It is proposed that very low template concentrations in the PCR generate random fluctuations in priming efficiency, which led to the contrast in the RFLP types observed in the libraries from the undiluted and diluted extracts.
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Towards a unified system for detecting waterborne pathogens
TL;DR: Recent advances in sample collection, on-line sample processing and purification, and DNA microarray technologies may form the basis of a universal method to detect known and emerging waterborne pathogens.
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Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays
TL;DR: The sensitivity and specificity of nucleic acid microarrays for detecting and genotyping E. coli O157:H7 were examined, with a glass-based array being found to be 32-fold more sensitive than gel electrophoresis and capable of detecting amplification products from < 1 cell equivalent of genomic DNA.
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Application of the 5′ Fluorogenic Exonuclease Assay (TaqMan) for Quantitative Ribosomal DNA and rRNA Analysis in Sediments
TL;DR: It is demonstrated that fluorescence quenching and autofluorescence can significantly affect Taq man PCR enumeration accuracy, with subsequent implications for the design and implementation of TaqMan PCR to sediments and related environmental samples.