David A. Widdick

TL;DR: The TatP method is able to discriminate Tat signal peptide from cytoplasmic proteins carrying a similar motif, as well as from Sec signal peptides, with high accuracy and generates far less false positive predictions on various datasets than using simple pattern matching.

TL;DR: In this paper, a comparison of the extracellular proteome of the wild type and DeltatatatC strains identified 73 predicted secretory proteins that were present in reduced amounts in the tatC mutant strain, and 47 Tat substrates were verified using a Tat reporter assay.

TL;DR: This report reports that Streptomyces species translocate large numbers of lipoproteins out via the Tat (twin arginine translocase) pathway and presents evidence that lipoprotein biogenesis might be an essential pathway in S. coelicolor and provides the first evidence thatlipoproteinBiogenesis could be essential in a Gram‐positive bacterium.

TL;DR: A detailed study of lipoprotein biogenesis in Streptomyces is presented, the first study of Lnt function in a monoderm bacterium and the firstStudy of bacterial lipoproteins as virulence factors in a plant pathogen is presented.

TL;DR: Time-lapse imaging revealed that localization of the Tat components was highly dynamic during tip growth and again demonstrated a strong preference for apical sites in growing hyphae, indicating repositioning of Tat components during the Streptomyces life cycle.