D
David M. Needham
Researcher at University of Southern California
Publications - 30
Citations - 4338
David M. Needham is an academic researcher from University of Southern California. The author has contributed to research in topics: 18S ribosomal RNA & Genome. The author has an hindex of 17, co-authored 30 publications receiving 2858 citations. Previous affiliations of David M. Needham include Auburn University & Monterey Bay Aquarium Research Institute.
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DatasetDOI
EMP 16S Illumina Amplicon Protocol
J. Greg Caporaso,Gail Ackermann,Amy Apprill,Markus J. Bauer,Donna Berg-Lyons,Jason Betley,Noah Fierer,Louise Fraser,Jed A. Fuhrman,Jack A. Gilbert,Niall Anthony Gormley,Greg Humphrey,James Huntley,Janet K. Jansson,Rob Knight,Chris Lauber,Catherine A. Lozupone,Sean McNally,David M. Needham,Sarah M. Owens,Alma E. Parada,Rachel Parsons,Geoff Smith,Luke R. Thompson,Luke Thompson,Peter J. Turnbaugh,William A. Walters,Laura Weber +27 more
Journal ArticleDOI
Comprehensive single-PCR 16S and 18S rRNA community analysis validated with mock communities, and estimation of sequencing bias against 18S.
TL;DR: In this paper, the authors developed 16S and 18S rRNA mock communities and a bioinformatic pipeline to validate this approach, using these mocks, they show that universal primers (515Y/926R) outperformed eukaryote-specific V4 primers in observed versus expected abundance correlations (slope = 0.88 vs. 0.67-0.79).
Journal ArticleDOI
Targeted metagenomic recovery of four divergent viruses reveals shared and distinctive characteristics of giant viruses of marine eukaryotes.
David M. Needham,Camille Poirier,Camille Poirier,Elisabeth Hehenberger,Elisabeth Hehenberger,Valeria Jimenez,Jarred E. Swalwell,Jarred E. Swalwell,Alyson E. Santoro,Alexandra Z. Worden,Alexandra Z. Worden +10 more
TL;DR: It is posited that the observed viral groupings are connected to host lifestyles as heterotrophs or phototrophing as well as other eukaryotic viruses, likely influencing the uptake of nitrogen during infection.
Journal ArticleDOI
Seasonal and Geographical Transitions in Eukaryotic Phytoplankton Community Structure in the Atlantic and Pacific Oceans.
Chang Jae Choi,Chang Jae Choi,Valeria Jimenez,David M. Needham,David M. Needham,Camille Poirier,Camille Poirier,Charles Bachy,Charles Bachy,Harriet Alexander,Harriet Alexander,Susanne Wilken,Susanne Wilken,Francisco P. Chavez,Sebastian Sudek,Stephen J. Giovannoni,Alexandra Z. Worden,Alexandra Z. Worden +17 more
TL;DR: Comparative analyses across the Pacific and Atlantic sites support the conclusion that predatory dictyochophytes thrive under low nutrient conditions, and raises questions about potential shifts in phytoplankton trophic roles associated with seasonality and long-term ocean change.
Journal ArticleDOI
Influence of Light on Particulate Organic Matter Utilization by Attached and Free-Living Marine Bacteria.
Laura Gómez-Consarnau,Laura Gómez-Consarnau,David M. Needham,Peter K. Weber,Jed A. Fuhrman,Xavier Mayali +5 more
TL;DR: Results show that light influences particle degradation and C and N incorporation by attached bacteria, suggesting that the transfer between particulate and free-living phases are likely affected by external factors that change with the light regime, such as time of day, water column depth and season.