David W. Piston

TL;DR: Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation.

TL;DR: No single GFP variant is ideal for every application, but each one offers advantages and disadvantages for quantitative imaging in living cells.

TL;DR: The physical basis of FRET and the fluorescent proteins appropriate for these experiments are described and the approaches that can be used to measure FRET are reviewed, with particular emphasis on the potential artifacts associated with each approach.

TL;DR: Evidence is provided that BMPs function during regional morphogenesis of the dorsal telencephalon by regulating specific gene expression, cell proliferation and local cell death.

TL;DR: It is shown that adipocyte-specific deletion of the coregulatory protein PRDM16 caused minimal effects on classical brown fat but markedly inhibited beige adipocyte function in subcutaneous fat following cold exposure or β3-agonist treatment, indicating that PRDM 16 and beige fat cells are required for the "browning" of white fat and the healthful effects of sub cutaneous adipose tissue.