D
Diana Machado
Researcher at Universidade Nova de Lisboa
Publications - 75
Citations - 2535
Diana Machado is an academic researcher from Universidade Nova de Lisboa. The author has contributed to research in topics: Mycobacterium tuberculosis & Efflux. The author has an hindex of 25, co-authored 71 publications receiving 1935 citations. Previous affiliations of Diana Machado include University of Lisbon.
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Journal ArticleDOI
Contribution of Efflux to the Emergence of Isoniazid and Multidrug Resistance in Mycobacterium tuberculosis
Diana Machado,Isabel Couto,João Perdigão,Liliana Rodrigues,Isabel Portugal,Pedro V. Baptista,Bruno Veigas,Leonard Amaral,Miguel Viveiros +8 more
TL;DR: Results support the hypothesis that activity of efflux pumps allows the maintenance of an isoniazid resistant population in a sub-optimally treated patient and should be considered in the development of new therapeutic strategies for preventing the emergence of MDR-TB during treatment.
Journal ArticleDOI
Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs.
Jody Phelan,Denise M. O'Sullivan,Diana Machado,Jorge Ramos,Yaa E. A. Oppong,Susana Campino,Justin O'Grady,Ruth McNerney,Martin L. Hibberd,Miguel Viveiros,Jim F. Huggett,Taane G. Clark,Taane G. Clark +12 more
TL;DR: A new command line version of the TBProfiler webserver is presented, which includes hetero-resistance calling and will facilitate the batch processing of samples and can rapidly and accurately predict anti-TB drug resistance profiles across large numbers of samples with WGS data.
Journal ArticleDOI
Contribution of efflux activity to isoniazid resistance in the Mycobacterium tuberculosis complex.
TL;DR: It is demonstrated that efflux pumps play an important role in acquired INH resistance in M. tuberculosis complex and the development of EIs that can restore the antimicrobial activity of the antibiotic subject to efflux is an approach that can be useful in order to prevent the emergence of this resistance and guide thedevelopment of new effective anti-TB therapeutical approaches.
Journal ArticleDOI
Exploring the contribution of efflux on the resistance to fluoroquinolones in clinical isolates of Staphylococcus aureus
Sofia Santos Costa,Celeste Falcão,Miguel Viveiros,Diana Machado,Marta Martins,Marta Martins,José Melo-Cristino,Leonard Amaral,Isabel Couto +8 more
TL;DR: The data gathered by real-time fluorometric and RT-qPCR assays suggest that S. aureus clinical isolates may be primed to efflux antimicrobial compounds, and underline the contribution of efflux systems for the emergence of high-level resistance.
Journal ArticleDOI
High-level resistance to isoniazid and ethionamide in multidrug-resistant Mycobacterium tuberculosis of the Lisboa family is associated with inhA double mutations
Diana Machado,João Perdigão,Jorge Ramos,Isabel Couto,Isabel Portugal,Claudia Ritter,Erik C. Boettger,Miguel Viveiros +7 more
TL;DR: The results reveal that the presence of a mutation in the inhA regulatory region together with a mutationIn inhA coding region can lead to the development of high-level isoniazid resistance and cross-resistance to ethionamide among the MDR-TB strains circulating in Lisbon.