Susana Campino

TL;DR: A robust and optimized Next-Generation Sequencing library amplification method suitable for extremely AT-rich genomes and will greatly benefit sequencing clinical samples that often require amplification due to low mass of DNA starting material is developed.

TL;DR: Analysis of the fine structure of the parasite population showed that the fd, arps10, mdr2 and crt polymorphisms are markers of a genetic background on which kelch13 mutations are particularly likely to arise and that they correlate with the contemporary geographical boundaries and population frequencies of artemisinin resistance.

TL;DR: Methods for the large-scale analysis of genetic variation in Plasmodium falciparum by deep sequencing of parasite DNA obtained from the blood of patients with malaria, either directly or after short-term culture are described.

TL;DR: An analysis of genome variation in 825 P. falciparum samples from Asia and Africa is described that identifies an unusual pattern of parasite population structure at the epicenter of artemisinin resistance in western Cambodia, and a catalog of SNPs that show high levels of differentiation in the art Artemisinin-resistant subpopulations are provided.

TL;DR: Findings identify PfAP2-G as a master regulator of sexual-stage development in malaria parasites and mark the first discovery of a transcriptional switch controlling a differentiation decision in protozoan parasites.