Diana Machado

TL;DR: Results support the hypothesis that activity of efflux pumps allows the maintenance of an isoniazid resistant population in a sub-optimally treated patient and should be considered in the development of new therapeutic strategies for preventing the emergence of MDR-TB during treatment.

TL;DR: A new command line version of the TBProfiler webserver is presented, which includes hetero-resistance calling and will facilitate the batch processing of samples and can rapidly and accurately predict anti-TB drug resistance profiles across large numbers of samples with WGS data.

TL;DR: It is demonstrated that efflux pumps play an important role in acquired INH resistance in M. tuberculosis complex and the development of EIs that can restore the antimicrobial activity of the antibiotic subject to efflux is an approach that can be useful in order to prevent the emergence of this resistance and guide thedevelopment of new effective anti-TB therapeutical approaches.

TL;DR: The data gathered by real-time fluorometric and RT-qPCR assays suggest that S. aureus clinical isolates may be primed to efflux antimicrobial compounds, and underline the contribution of efflux systems for the emergence of high-level resistance.

TL;DR: The results reveal that the presence of a mutation in the inhA regulatory region together with a mutationIn inhA coding region can lead to the development of high-level isoniazid resistance and cross-resistance to ethionamide among the MDR-TB strains circulating in Lisbon.