Dick Hoekstra

TL;DR: The data suggest that improvement of non-viral gene transfection could very much benefit from controlling particle size, which would allow targeting of particle internalization via a non-degradative pathway, involving caveolae-mediated endocytosis.

TL;DR: In this paper, double-chained pyridinium compounds have been developed that display highly efficient DNA transfection properties, and the authors demonstrate that the enhanced efficiency relates to the fact that a relative higher number of cells in the population is transfected (≈50% in the case of COS cells) by the pyrinium derivatives, whereas the delivery of DNA per cell is also enhanced.

TL;DR: This review gives a concise overview of studies on the application of cationic liposomes in vivo in animal models of diseases and in clinical studies, and discusses the transfection efficiency, the pharmacokinetic and pharmacodynamic properties of the lipid-DNA complexes, and potentially relevant applications for cationo- DNA complexes.

TL;DR: The fusion experiments performed in the presence of poly(ethylene glycol) suggest that the degree of bilayer dehydration and the creation of "point defects" in the bilayer without rigorous structural rearrangements in the membrane are dominant factors in the initial fusion events.

TL;DR: It is concluded that in the DOPE-containing complexes the molecular shape and the nonbilayer preferences of the cationic lipid control the topology of the lipoplex and thereby the transfection efficiency.