Dick Hoekstra

TL;DR: Under conditions where leakage of vesicle contents during fusion is limited, both fusion assays accurately reflect the occurrence of this process, thus indicating the usefulness of these assays as reliable, complementary tools in the study of fusion of (model) membranes.

TL;DR: Transfection efficiency seems to require a positive or neutral zeta potential, which is depending on size, e.g., is higher for smaller particles, and requires a vector that is stable in serum.

TL;DR: The kinetics of the R18-labeled lipid mixing were compared to those obtained with an assay in which the fusion-monitoring probe, eosin-maleimide, was attached to the viral surface proteins and the increase in R18 fluorescence was evaluated in terms of the different processes that in principle may contribute to this increase.

TL;DR: Biodegradable synthetic cationic pyridinium-based amphiphiles (SAINTs) prove to be promising non-viral carrier systems for delivery of DNA into eukaryotic cells.

TL;DR: This study provides an optimized 3D model system for the integrated analysis of (real-time) intestinal epithelial paracellular permeability and morphogenesis, and reveals apoptosis as a pivotal mechanism underlying the enhanced permeable and altered morphogenesis in response to TNF, but not IFNγ.