D
Dirk Valckx
Researcher at Catholic University of Leuven
Publications - 25
Citations - 3881
Dirk Valckx is an academic researcher from Catholic University of Leuven. The author has contributed to research in topics: NOD mice & Immune system. The author has an hindex of 17, co-authored 25 publications receiving 3742 citations. Previous affiliations of Dirk Valckx include Katholieke Universiteit Leuven.
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Journal ArticleDOI
An overview of real-time quantitative PCR: applications to quantify cytokine gene expression.
Annapaula Giulietti,Lutgart Overbergh,Dirk Valckx,Brigitte Decallonne,Roger Bouillon,Chantal Mathieu +5 more
TL;DR: The real-time RT-PCR technique is very accurate and sensitive, allows a high throughput, and can be performed on very small samples; therefore it is the method of choice for quantification of cytokine profiles in immune cells or inflamed tissues.
Journal ArticleDOI
Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR.
TL;DR: Data validating this PCR-method for the quantification of murine cytokines and other factors playing a role in immune regulation is presented, avoiding time-consuming and hazardous post-PCR manipulations and decreasing the potential risk of PCR contamination.
Journal Article
The Use of Real-Time Reverse Transcriptase PCR for the Quantification of Cytokine Gene Expression
Lutgart Overbergh,Annapaula Giulietti,Dirk Valckx,Brigitte Decallonne,Roger Bouillon,Chantal Mathieu +5 more
TL;DR: The development of real-time RT-PCR has resulted in an exponential increase in its use over the last couple of years, and the method has undoubtedly become the standard for quantifying cytokine patterns, clarifying many functional properties of immune cells and their associated diseases.
Journal ArticleDOI
Identification and immune regulation of 25-hydroxyvitamin D-1-alpha-hydroxylase in murine macrophages.
Lutgart Overbergh,Brigitte Decallonne,Dirk Valckx,Annemieke Verstuyf,Jos Depovere,Jozef Laureys,Omer Rutgeerts,Rene Saint-Arnaud,Roger Bouillon,Chantal Mathieu +9 more
TL;DR: It is proposed that the up‐regulation of 1‐α‐hydroxylase in activated macrophages, resulting in the synthesis of 1,25(OH)2D3, might be a negative feedback loop in inflammation.
Journal ArticleDOI
1alpha,25-dihydroxyvitamin D3 induces an autoantigen-specific T-helper 1/T-helper 2 immune shift in NOD mice immunized with GAD65 (p524-543).
Lutgart Overbergh,Brigitte Decallonne,Mark Waer,Omer Rutgeerts,Dirk Valckx,Kristina Casteels,Jozef Laureys,Roger Bouillon,Chantal Mathieu +8 more
TL;DR: The immune deviation induced by 1alpha,25(OH)2D3 in NOD mice can also be induced in the peripheral immune system but is limited to pancreatic autoantigens.