L
Lutgart Overbergh
Researcher at Katholieke Universiteit Leuven
Publications - 83
Citations - 7526
Lutgart Overbergh is an academic researcher from Katholieke Universiteit Leuven. The author has contributed to research in topics: Cytokine & NOD mice. The author has an hindex of 39, co-authored 75 publications receiving 7111 citations. Previous affiliations of Lutgart Overbergh include National Fund for Scientific Research & Catholic University of Leuven.
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Journal ArticleDOI
An overview of real-time quantitative PCR: applications to quantify cytokine gene expression.
Annapaula Giulietti,Lutgart Overbergh,Dirk Valckx,Brigitte Decallonne,Roger Bouillon,Chantal Mathieu +5 more
TL;DR: The real-time RT-PCR technique is very accurate and sensitive, allows a high throughput, and can be performed on very small samples; therefore it is the method of choice for quantification of cytokine profiles in immune cells or inflamed tissues.
Journal ArticleDOI
Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR.
TL;DR: Data validating this PCR-method for the quantification of murine cytokines and other factors playing a role in immune regulation is presented, avoiding time-consuming and hazardous post-PCR manipulations and decreasing the potential risk of PCR contamination.
Journal ArticleDOI
Initiation and execution of lipotoxic ER stress in pancreatic beta-cells.
Daniel Andrade Da Cunha,Paul Hekerman,Laurence Ladrière,Angie Bazarra-Castro,Fernanda Ortis,Marion C. Wakeham,Fabrice Moore,Joanne Rasschaert,Alessandra K Cardozo,Elisa A. Bellomo,Lutgart Overbergh,Chantal Mathieu,Roberto Lupi,Tsonwin Hai,André Herchuelz,Piero Marchetti,Guy A. Rutter,Decio L. Eizirik,Miriam Cnop,Miriam Cnop +19 more
TL;DR: Saturated FFA induce ER stress via ER Ca2+ depletion and the IRE1 and resulting JNK activation contribute to β-cell apoptosis, suggesting that ER stress in primary β-cells is primarily lipotoxic, and not glucolipotoxic.
Journal ArticleDOI
Interleukin-17 Orchestrates the Granulocyte Influx into Airways after Allergen Inhalation in a Mouse Model of Allergic Asthma
Peter Hellings,Ahmad Kasran,Zhanju Liu,Philippe Vandekerckhove,Anja Wuyts,Lutgart Overbergh,Chantal Mathieu,Jan Ceuppens +7 more
TL;DR: It is demonstrated that IL-17 expression in airways is upregulated upon allergen inhalation, and constitutes the link between allergenic-induced T cell activation and neutrophilic influx.
Journal Article
The Use of Real-Time Reverse Transcriptase PCR for the Quantification of Cytokine Gene Expression
Lutgart Overbergh,Annapaula Giulietti,Dirk Valckx,Brigitte Decallonne,Roger Bouillon,Chantal Mathieu +5 more
TL;DR: The development of real-time RT-PCR has resulted in an exponential increase in its use over the last couple of years, and the method has undoubtedly become the standard for quantifying cytokine patterns, clarifying many functional properties of immune cells and their associated diseases.