Dorte Møller Larsen

TL;DR: The work thus demonstrated the workability of in silico based screening coupled with a synthetic biology approach for gene synthesis for identification and production of a thermostable enzyme.

TL;DR: Proof of the concept of producing 3'-sialyllactose and potentially other sIALylated HMOs as well as sialylated GOS enzymatically by trans-sialidase activity is provided, while at the same time providing valorisation of CGMP, a co-processing product from cheese manufacture.

TL;DR: Casein glycomacropeptide and lactose were used as substrates for enzyme catalyzed production of 3′-sialyllactose, a model case compound for human milk oligosaccharides (HMOs) demonstrating the versatility of the Tr6 trans -sialidase for catalyzing sialyl-transfer for generating different HMOs.

TL;DR: The objective of the present study was to characterize and compare four homogalacturonan degrading enzymes capable of catalyzing the required solubilization of these pectinaceous polysaccharides from potato pulp in a 1 min reaction.

TL;DR: Rational engineering of Trypanosoma rangeli sialidase is reported to develop an effective enzyme for a potentially important type of reactivity: production of sialylated prebiotic glycans and it is suggested that this motif can serve as a framework for future optimization of trans-sialylation towards prebiotics.