Douglas A. Stirling

TL;DR: Evidence that DNA supercoiling plays a key role in the osmotic induction of proU transcription is presented, and mutations in osmZ are highly pleiotropic, affecting expression of a variety of chromosomal genes including ompF, ompC, fimA, and the bgl operon.

TL;DR: Transcription of the genes encoding the high-affinity potassium and betaine transport systems of E.coli and Salmonella typhimurium is tightly regulated in response to osmotic stress, and cytoplasmic ionic strength plays an important regulatory role.

TL;DR: Evidence is presented which suggests that, besides de novo glycine betaine uptake, an important function of ProU may be the recapture and recycling of other osmolytes that leak from the cell.

TL;DR: Overexpression of calmodulin suppresses the defect(s) associated with the mutant Spc110 proteins, supporting the notion that Spc 110p stability is a consequence of its ability to bind calmod insulin and pointing to a direct role for calmodul in Spc109p function.

TL;DR: A novel promoter probe plasmid pSB40 is constructed, containing a unique lac‐α‐tetracycline marker gene tandem, which allows for both positive and negative selection of active promoters in E. coli regulated by osmotic upshift.