Peng Qiu

TL;DR: Single-cell “mass cytometry” analyses provide system-wide views of immune signaling in healthy human hematopoiesis, against which drug action and disease can be compared for mechanistic studies and pharmacologic intervention.

TL;DR: This work presents a versatile computational approach, spanning-tree progression analysis of density-normalized events (SPADE), which facilitates the analysis of cellular heterogeneity, the identification of cell types and comparison of functional markers in response to perturbations.

TL;DR: The steps required to create high-quality image-based (i.e., morphological) profiles from a collection of microscopy images are introduced and techniques that have proven useful in each stage of the data analysis process are recommended on the basis of the experience of 20 laboratories worldwide that are refining their image- based cell-profiling methodologies.

TL;DR: TCGA-Assembler is introduced, a software package that automates and streamlines the retrieval, assembly, and processing of public TCGA data and opens a door for the development of data-mining and data-analysis tools that generate fully reproducible results, including data acquisition.

TL;DR: A bioinformatics-based tool, COSMID (CRISPR Off-target Sites with Mismatches, Insertions, and Deletions) that searches genomes for potential off-target sites, thus helping the design of CRISPR/Cas systems with minimal off- target effects, as well as the identification and quantification of CRispr/Cas induced off- Target cleavage in cells.