Elisabeth Fischer-Friedrich

TL;DR: Real-time deformability cytometry (RT-DC) is introduced for continuous cell mechanical characterization of large populations with analysis rates greater than 100 cells/s and adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

TL;DR: A reaction-diffusion model of the MinD and MinE dynamics is presented that accounts for the experimental observations and also captures the in vivo oscillations and formed planar surface waves on a flat membrane in vitro.

TL;DR: This work disentangle mutual contributions of cell size and cell stiffness to cell deformation by a theoretical analysis in terms of hydrodynamics and linear elasticity theory and demonstrates that the analytical model not only predicts deformed shapes inside the channel but also allows for quantification of cell mechanical parameters.

TL;DR: In this paper, the authors characterize the time-dependent material properties of in vitro protein condensates using laser tweezer-based active and microbead-based passive rheology.

TL;DR: It is proposed that protein detachment at the rear of the wave, and the formation of the E-ring, are accomplished by two complementary processes: first, local accumulation of MinE due to rapid rebinding, leading to dynamic instability; and second, a structural change induced by membrane-interaction of Min E in an equimolar MinD–MinE (MinDE) complex, which supports the robustness of pattern formation.