Daniel Klaue

TL;DR: Real-time deformability cytometry (RT-DC) is introduced for continuous cell mechanical characterization of large populations with analysis rates greater than 100 cells/s and adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

TL;DR: It is found that for a kinked DNA molecule the abrupt extension change occurs at significantly lower twist than the subsequent superhelix formation, which should allow pinning of the plectoneme position within supercoiled DNA if a kinky substrate is used, and enable the detection of enzymes and proteins which, themselves, bend or kink DNA.

TL;DR: This work demonstrates that camera-based imaging can provide a similar performance for all three dimensions of particle tracking with Ångström accuracy as laser detection through photodiodes, and provides a simple and robust way for high-resolution tweezers experiments using multiple particles at a time.

TL;DR: By investigating the axial fluctuations of DNA-bound microspheres, it is found that considerable rotational microsphere fluctuations can occur and Quantitative noise analysis allowed us to determine the rotational stiffness of individual micro spheres, which was found to saturate at high magnetic fields.

TL;DR: The nuclease of Dna2 inhibits its helicase by cleaving 5′ flaps that are required by the helicase domain for loading onto its substrate, which limits and controls the enzyme's capacity to unwind dsDNA.