E
Ellen Nordman
Researcher at Applied Biosystems
Publications - 4
Citations - 3842
Ellen Nordman is an academic researcher from Applied Biosystems. The author has contributed to research in topics: Transcriptome & Gene expression profiling. The author has an hindex of 4, co-authored 4 publications receiving 3110 citations.
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Journal ArticleDOI
mRNA-Seq whole-transcriptome analysis of a single cell.
Fuchou Tang,Catalin Barbacioru,Yangzhou Wang,Ellen Nordman,Clarence Lee,Nanlan Xu,Xiaohui Wang,John Bodeau,Brian B. Tuch,Asim Siddiqui,Kaiqin Lao,M. Azim Surani +11 more
TL;DR: A single-cell digital gene expression profiling assay with only a single mouse blastomere is described, which detected the expression of 75% more genes than microarray techniques and identified 1,753 previously unknown splice junctions called by at least 5 reads.
Journal ArticleDOI
Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNA-Seq analysis
Fuchou Tang,Catalin Barbacioru,Siqin Bao,Caroline Lee,Ellen Nordman,Xiaohui Wang,Kaiqin Lao,M. Azim Surani +7 more
TL;DR: Significant molecular transitions and major changes in transcript variants are detected, which include genes for general metabolism and changes in microRNAs, which may contribute to a switch from a normal developmental program in adult cells during the formation of diseased tissues, including cancers.
Journal ArticleDOI
RNA-Seq analysis to capture the transcriptome landscape of a single cell
Fuchou Tang,Catalin Barbacioru,Ellen Nordman,Bin Li,Nanlan Xu,Vladimir I. Bashkirov,Kaiqin Lao,M. Azim Surani +7 more
TL;DR: This protocol can generate deep-sequencing libraries for 16 single-cell samples within 6 d and can capture up to 75% more genes expressed in early embryos compared with cDNA microarray techniques.
Journal ArticleDOI
Deterministic and Stochastic Allele Specific Gene Expression in Single Mouse Blastomeres
Fuchou Tang,Catalin Barbacioru,Ellen Nordman,Siqin Bao,Caroline Lee,Xiaohui Wang,Brian B. Tuch,Edith Heard,Kaiqin Lao,M. Azim Surani +9 more
TL;DR: It is demonstrated that ASE is both deterministic and stochastic in early blastomeres, and 1,718 genes express two isoforms with different lengths of 3′UTRs, suggesting that microRNA mediated regulation of gene expression acquires increasing importance as development progresses.