E
Emil F. Pai
Researcher at University of Toronto
Publications - 203
Citations - 15866
Emil F. Pai is an academic researcher from University of Toronto. The author has contributed to research in topics: Binding site & Xanthine dehydrogenase. The author has an hindex of 58, co-authored 203 publications receiving 14840 citations. Previous affiliations of Emil F. Pai include Toronto General Hospital & University of Georgia.
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Journal ArticleDOI
Crystallographic studies on p21H−ras using the synchrotron Laue method: improvement of crystal quality and monitoring of the GTPase reaction at different time points
Axel J. Scheidig,Antonieta Sanchez-Llorente,Alfred Lautwein,Emil F. Pai,John E. T. Corrie,Gordon P. Reid,Alfred Wittinghofer,Roger S. Goody +7 more
TL;DR: The refinement of five data sets collected within 2 min at different times after the initiation of the intrinsic GTPase reaction of the protein indicates that the synchrotron Laue method can be used to detect small structural changes and alternative conformations, but is presently limited in the analysis of larger rearrangements since these produce diffuse and broken electron density.
Journal ArticleDOI
Crystal structure of the complex between the F(ab)' fragment of the cross-neutralizing anti-HIV-1 antibody 2F5 and the F(ab) fragment of its anti-idiotypic antibody 3H6.
Steve Bryson,Jean-Philippe Julien,David E. Isenman,Renate Kunert,Hermann Katinger,Emil F. Pai +5 more
TL;DR: Ab2/3H6 is an anti-idiotypic antibody of the Ab2gamma class, i.e., an antigen-inhibitable idiotype that does not carry the internal image of the linear primary gp41 (662)ELDKWAS(668) epitope.
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Purification, crystallization and preliminary X-ray study of orotidine 5′-monophosphate decarboxylase
TL;DR: Orotidine-5'-monophosphate decarboxylase (ODCase) from Methanobacterium thermoautotrophicum has been crystallized with and without the inhibitor 6-azaUMP by the vapour-diffusion method.
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Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison.
Pedram Mehrabi,Robert Bücker,Robert Bücker,Gleb Bourenkov,Helen M. Ginn,D. von Stetten,Henrike M. Müller-Werkmeister,A. Kuo,Takefumi Morizumi,Bryan T. Eger,Wei-Lin Ou,Saeed Oghbaey,Antoine Sarracini,Jessica E. Besaw,O. Pare´-Labrosse,O. Pare´-Labrosse,S. Meier,H. Schikora,Friedjof Tellkamp,Andreas Marx,D.A. Sherrell,Danny Axford,Robin L. Owen,Oliver P. Ernst,Emil F. Pai,Emil F. Pai,Eric Schulz,R.J.D. Miller +27 more
TL;DR: The direct comparability of SSX and SFX data indicates that diffraction quality is rather linked to the properties of the crystals than to the radiation source, and time-resolved experiments can be conducted at the source that best matches the desired time-resolution.
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Crystal structure of alkyl hydroperoxidase D like protein PA0269 from Pseudomonas aeruginosa: Homology of the AhpD-like structural family
Teresa E. Clarke,Vladimir Romanov,Yuri N. Chirgadze,Chananat Klomsiri,Gera Kisselman,Jean Wu-Brown,Leslie B. Poole,Emil F. Pai,Emil F. Pai,Nickolay Y. Chirgadze,Nickolay Y. Chirgadze +10 more
TL;DR: It is shown that AhpD from P. aeruginosa exhibits a weak ability to reduce H2O2 as tested using a ferrous oxidation-xylenol orange (FOX) assay, and this activity is blocked by thiol alkylating reagents.