F
Feng Lan
Researcher at Capital Medical University
Publications - 101
Citations - 5883
Feng Lan is an academic researcher from Capital Medical University. The author has contributed to research in topics: Induced pluripotent stem cell & Stem cell. The author has an hindex of 30, co-authored 87 publications receiving 4789 citations. Previous affiliations of Feng Lan include Peking Union Medical College & Stanford University.
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Journal ArticleDOI
Chemically defined generation of human cardiomyocytes
Paul W. Burridge,Elena Matsa,Praveen K. Shukla,Ziliang C Lin,Jared M. Churko,Antje D. Ebert,Feng Lan,Sebastian Diecke,Bruno C. Huber,Nicholas M. Mordwinkin,Jordan R. Plews,Oscar J. Abilez,Bianxiao Cui,Joseph D. Gold,Joseph C. Wu +14 more
TL;DR: This work systematically developed an optimized cardiac differentiation strategy, using a chemically defined medium consisting of just three components: the basal medium RPMI 1640, L-ascorbic acid 2-phosphate and rice-derived recombinant human albumin, which was effective in 11 hiPSC lines tested.
Journal ArticleDOI
Abnormal Calcium Handling Properties Underlie Familial Hypertrophic Cardiomyopathy Pathology in Patient-Specific Induced Pluripotent Stem Cells
Feng Lan,Andrew S. Lee,Ping Liang,Veronica Sanchez-Freire,Patricia K. Nguyen,Li Wang,Leng Han,Michelle Yen,Yongming Wang,Ning Sun,Oscar J. Abilez,Shijun Hu,Antje D. Ebert,Enrique G. Navarrete,Chelsey S. Simmons,Matthew T. Wheeler,Beth L. Pruitt,Richard S. Lewis,Yoshinori Yamaguchi,Euan A. Ashley,Donald M. Bers,Robert C. Robbins,Michael T. Longaker,Joseph C. Wu +23 more
TL;DR: Patients generating patient-specific induced pluripotent stem cell cardiomyocytes from a ten-member family cohort carrying a hereditary HCM missense mutation (Arg663His) in the MYH7 gene are generated to help elucidate the mechanisms underlying HCM development and identify novel therapies for the disease.
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Drug Screening Using a Library of Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes Reveals Disease-Specific Patterns of Cardiotoxicity
Ping Liang,Feng Lan,Andrew S. Lee,Tingyu Gong,Veronica Sanchez-Freire,Yongming Wang,Sebastian Diecke,Karim Sallam,Joshua W. Knowles,Paul J. Wang,Patricia K. Nguyen,Donald M. Bers,Robert C. Robbins,Joseph C. Wu +13 more
TL;DR: The data indicate that healthy and diseased individuals exhibit different susceptibilities to cardiotoxic drugs and that use of disease-specific hiPSC-CMs may predict adverse drug responses more accurately than the standard human ether-a-go-go–related gene test or healthy control hiPSc-CM/hESC-CM screening assays.
Journal ArticleDOI
Screening drug-induced arrhythmia events using human induced pluripotent stem cell-derived cardiomyocytes and low-impedance microelectrode arrays
Enrique G. Navarrete,Ping Liang,Feng Lan,Veronica Sanchez-Freire,Chelsey S. Simmons,Tingyu Gong,Arun Sharma,Paul W. Burridge,Bhagat Patlolla,Andrew S. Lee,Haodi Wu,Ramin E. Beygui,Sean M. Wu,Robert C. Robbins,Donald M. Bers,Joseph C. Wu +15 more
TL;DR: The data indicate that the MEA/hiPSC-CM assay is a sensitive, robust, and efficient platform for testing drug effectiveness and for arrhythmia screening and may provide significant advantages over current industry standard assays that use immortalized cell lines or animal models.
Journal ArticleDOI
Patient-Specific and Genome-Edited Induced Pluripotent Stem Cell–Derived Cardiomyocytes Elucidate Single-Cell Phenotype of Brugada Syndrome
Ping Liang,Karim Sallam,Haodi Wu,Yingxin Li,Ilanit Itzhaki,Priyanka Garg,Ying Zhang,Vittavat Vermglinchan,Feng Lan,Mingxia Gu,Tingyu Gong,Tingyu Gong,Yan Zhuge,Chunjiang He,Antje D. Ebert,Veronica Sanchez-Freire,Jared M. Churko,Shijun Hu,Arun Sharma,Chi Keung Lam,Melvin M. Scheinman,Donald M. Bers,Joseph C. Wu +22 more
TL;DR: Patient-specific iPSC-CMs were able to recapitulate single-cell phenotype features of Brugada syndrome, including blunted inward sodium current, increased triggered activity, and abnormal Ca2+ handling.