F
Florence M. Chardon
Researcher at University of Washington
Publications - 9
Citations - 209
Florence M. Chardon is an academic researcher from University of Washington. The author has contributed to research in topics: Computer science & Medicine. The author has an hindex of 3, co-authored 5 publications receiving 17 citations.
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Journal ArticleDOI
Precise genomic deletions using paired prime editing.
Junhong Choi,Junhong Choi,Wei Chen,Chase C. Suiter,Choli Lee,Florence M. Chardon,Wei Yang,Anh Leith,Riza M. Daza,Beth Martin,Jay Shendure +10 more
TL;DR: In this paper, a prime editing-based method, called PRIME-Del, was proposed to delete genomic sequences up to 10kb using a pair of prime editing sgRNAs (pegRNAs) targeting opposite DNA strands.
Journal ArticleDOI
High-content CRISPR screening
Christopher J Bock,Paul Datlinger,Florence M. Chardon,Matthew A. Coelho,Matthew B. Dong,Keith A. Lawson,Tian Lu,Laetitia Maroc,Thomas M. Norman,Bicna Song,Geoff Stanley,Sidi Chen,Mathew J. Garnett,Wei Li,Jason Moffat,Lei S. Qi,Rebecca S. Shapiro,Jay Shendure,Jonathan S. Weissman,Xiao-Xi Zhuang +19 more
Journal ArticleDOI
A time-resolved, multi-symbol molecular recorder via sequential genome editing
Junhong Choi,Wei-Ji Chen,Anna Minkina,Florence M. Chardon,Chase C. Suiter,Samuel G. Regalado,Silvia Domcke,Nobuhiko Hamazaki,Choli Lee,Beth Martin,Riza M. Daza,Jay Shendure +11 more
TL;DR: The DNA Typewriter as mentioned in this paper is a general system for in vivo molecular recording that overcomes the limitations of DNA-based memory devices in terms of the number of distinct symbols that can be concurrently recorded and/or by a failure to capture the order in which events occur.
Posted ContentDOI
TransMPRA: A framework for assaying the role of many trans-acting factors at many enhancers
Diego Calderon,Andria Ellis,Riza M. Daza,Beth Martin,Jacob M. Tome,Wei Chen,Florence M. Chardon,Anh Leith,Choli Lee,Cole Trapnell,Jay Shendure +10 more
TL;DR: A reporter assay that efficiently combines multiplex CRISPR-mediated perturbation and MPRAs to identify trans-acting factors that modulate the regulatory activity of specific enhancers is described.
Posted ContentDOI
A temporally resolved, multiplex molecular recorder based on sequential genome editing
Junhong Choi,Junhong Choi,Wei Chen,Anna Minkina,Florence M. Chardon,Chase C. Suiter,Samuel G. Regalado,Silvia Domcke,Nobuhiko Hamazaki,Nobuhiko Hamazaki,Choli Lee,Beth Martin,Riza M. Daza,Jay Shendure +13 more
TL;DR: The DNA Ticker Tape as discussed by the authors is a general system for in vivo molecular recording that largely overcomes the limitations of DNA-based memory devices described to date, which are constrained in terms of the number of distinct signals that can be concurrently recorded and/or by a failure to capture the precise order of recorded events.