J
Jason Moffat
Researcher at University of Toronto
Publications - 226
Citations - 23547
Jason Moffat is an academic researcher from University of Toronto. The author has contributed to research in topics: Gene & Biology. The author has an hindex of 58, co-authored 195 publications receiving 19301 citations. Previous affiliations of Jason Moffat include Canadian Institute for Advanced Research & Merck & Co..
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Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen
Jason Moffat,Dorre A. Grueneberg,Xiaoping Yang,So Young Kim,So Young Kim,Angela M. Kloepfer,Gregory Hinkle,Gregory Hinkle,Bruno Piqani,Thomas Eisenhaure,Biao Luo,Jennifer K. Grenier,Anne E. Carpenter,Shi Yin Foo,Sheila A. Stewart,Brent R. Stockwell,Nir Hacohen,Nir Hacohen,William C. Hahn,William C. Hahn,Eric S. Lander,David M. Sabatini,David M. Sabatini,David E. Root +23 more
TL;DR: A screen based on high-content imaging was developed to identify genes required for mitotic progression in human cancer cells and applied to an arrayed set of 5,000 unique shRNA-expressing lentiviruses that target 1,028 human genes, providing a widely applicable resource for loss-of-function screens.
Journal ArticleDOI
Ablation in Mice of the mTORC Components raptor, rictor, or mLST8 Reveals that mTORC2 Is Required for Signaling to Akt-FOXO and PKCα, but Not S6K1
David A. Guertin,David A. Guertin,Deanna Stevens,Carson C. Thoreen,Aurora A. Burds,Nada Y. Kalaany,Jason Moffat,Jason Moffat,Michael S. Brown,Kevin Fitzgerald,David M. Sabatini,David M. Sabatini +11 more
TL;DR: MTORC1 function is essential in early development, mLST8 is required only for mTORC2 signaling, and m TORC2 is a necessary component of the Akt-FOXO and PKCalpha pathways.
Journal ArticleDOI
High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities
Traver Hart,Megha Chandrashekhar,Michael Aregger,Zachary Steinhart,Kevin R. Brown,Graham MacLeod,Monika Mis,Michal Zimmermann,Amélie Fradet-Turcotte,Song Sun,Patricia Mero,Peter B. Dirks,Sachdev S. Sidhu,Frederick P. Roth,Olivia S. Rissland,Daniel Durocher,Daniel Durocher,Stephane Angers,Jason Moffat,Jason Moffat +19 more
TL;DR: It is demonstrated that context-dependent fitness genes accurately recapitulate pathway-specific genetic vulnerabilities induced by known oncogenes and reveal cell-type-specific dependencies for specific receptor tyrosine kinases, even in oncogenic KRAS backgrounds.
Journal ArticleDOI
Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate
Gregory Huyer,Susana Liu,John F. Kelly,Jason Moffat,Paul Payette,Brian K. Kennedy,George Tsaprailis,Michael J. Gresser,Chidambaram Ramachandran +8 more
TL;DR: The results show that vanadate is a competitive inhibitor for the protein-tyrosine phosphatase PTP1B, with a Ki of 0.38 ± 0.02 μM, and reducing agents such as dithiothreitol that are used in PTP assays to keep the catalytic cysteine reduced and active were found to convert pervanadate rapidly toVanadate.