Journal ArticleDOI
High-content CRISPR screening
Christopher J Bock,Paul Datlinger,Florence M. Chardon,Matthew A. Coelho,Matthew B. Dong,Keith A. Lawson,Tian Lu,Laetitia Maroc,Thomas M. Norman,Bicna Song,Geoff Stanley,Sidi Chen,Mathew J. Garnett,Wei Li,Jason Moffat,Lei S. Qi,Rebecca S. Shapiro,Jay Shendure,Jonathan S. Weissman,Xiao-Xi Zhuang +19 more
Reads0
Chats0
About:
This article is published in Nature Reviews Methods Primers.The article was published on 2022-02-10. It has received 61 citations till now. The article focuses on the topics: Medicine & CRISPR.read more
Citations
More filters
Journal ArticleDOI
CRISPR technology: A decade of genome editing is only the beginning
Joy Y. Wang,Jennifer A. Doudna +1 more
TL;DR: Wang et al. as mentioned in this paper reviewed the origins and utility of CRISPR-based genome editing, the successes and current limitations of the technology, and where innovation and engineering are needed.
Journal ArticleDOI
Prime editing for precise and highly versatile genome manipulation
Peter J. Chen,David R. Liu +1 more
Journal ArticleDOI
Advancing CAR T cell therapy through the use of multidimensional omics data
Journal ArticleDOI
Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors
Joseph M. Replogle,Jessica L. Bonnar,Angela N. Pogson,Christina R. Liem,Nolan K. Maier,Yufang Ding,Baylee J. Russell,Xingren Wang,Kun Leng,Ali Guna,Thomas M. Norman,Ryan A. Pak,Daniel M. Ramos,Michael E. Ward,Luke A. Gilbert,Martin Kampmann,Jonathan S. Weissman,Marco Jost +17 more
TL;DR: This work combines empirical sgRNA selection with a dual-sgRNA library design to generate an ultra-compact, highly active, and rigorously compare CRISPRi effectors to show that the recently published Zim3-dCas9 provides the best balance between strong on-target knockdown and minimal nonspecific effects on cell growth or the transcriptome.
Journal ArticleDOI
Carbon-negative synthetic biology: challenges and emerging trends of cyanobacterial technology.
Chun Hung Tan,Ping Xu,Fei Tao +2 more
TL;DR: In this paper , the authors summarize recent advances in the production of value-added chemicals using cyanobacterial cell factories, particularly in carbon-negative synthetic biology and emerging trends in cyanobacteria applications.
References
More filters
Journal ArticleDOI
Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Comprehensive Integration of Single-Cell Data.
Tim Stuart,Andrew Butler,Paul J. Hoffman,Christoph Hafemeister,Efthymia Papalexi,William M. Mauck,Yuhan Hao,Marlon Stoeckius,Peter Smibert,Rahul Satija +9 more
TL;DR: A strategy to "anchor" diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.
Journal ArticleDOI
Enrichr: a comprehensive gene set enrichment analysis web server 2016 update
Maxim V. Kuleshov,Matthew R. Jones,Andrew D. Rouillard,Nicolas F. Fernandez,Qiaonan Duan,Zichen Wang,Simon Koplev,Sherry L. Jenkins,Kathleen M. Jagodnik,Alexander Lachmann,Michael G. McDermott,Caroline D. Monteiro,Gregory W. Gundersen,Avi Ma'ayan +13 more
TL;DR: A significant update to one of the tools in this domain called Enrichr, a comprehensive resource for curated gene sets and a search engine that accumulates biological knowledge for further biological discoveries is presented.
Related Papers (5)
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array
Bernd Zetsche,Matthias Heidenreich,Prarthana Mohanraju,Iana Fedorova,Jeroen Kneppers,Jeroen Kneppers,Ellen M DeGennaro,Ellen M DeGennaro,Nerges Winblad,Sourav R Choudhury,Omar O. Abudayyeh,Jonathan S. Gootenberg,Wen Y Wu,David A. Scott,David A. Scott,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,John van der Oost,Feng Zhang +19 more
Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
Bernd Zetsche,Matthias Heidenreich,Prarthana Mohanraju,Iana Fedorova,Jeroen Kneppers,Jeroen Kneppers,Ellen M DeGennaro,Ellen M DeGennaro,Nerges Winblad,Sourav R Choudhury,Omar O. Abudayyeh,Jonathan S. Gootenberg,Wen Y Wu,David A. Scott,David A. Scott,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,John van der Oost,Feng Zhang +19 more