Showing papers in "Cryobiology in 1998"

TL;DR: A prime need in cryosurgery research is related to the periphery of the cryosurgical lesion where some cells die and others live, and adjunctive therapy should influence the fate of cells in this region and increase the efficacy of cryos surgical techniques.

TL;DR: This paper demonstrates that the 2P and KK formalism yield essentially the same result (Lp and Ps) when cotransporting channels are absent, and discusses the phenomenological nature of transport parameters in many experiments, especially when both bilayer and channel transport are present.

TL;DR: The glycerol/trehalose combination provides a defined cryoprotectant which, when used with addition by dialysis prefreeze and direct insemination postthaw, yields a satisfactory yield of fertilized eggs in an in vitro fertilization system.

TL;DR: It is demonstrated that the high concentration of sodium in conventional freezing media is detrimental to oocyte cryopreservation and show that choline is a promising replacement.

TL;DR: This DSC technique is a promising new approach for measuring water transport in cellular systems during freezing and the decrease in heat release from osmotically active cells measured by the DSC during repetitive freezing and thawing was found to correlate strongly with the viability of the cells measured during identical freeze/thaw protocols with cryomicroscopy.

TL;DR: This paper reviews knowledge, most of which has been acquired in the last 10 years, in the field of cold stress responses and hopes that these data will help to focus attention on the metabolic responses associated with environmental disturbance.

TL;DR: The results of cultivation suggest that the gradual saturation/removal of cryoprotectant, elevated temperature, and pretreatment with cytoskeletal inhibitor Cytochalasin B have a positive effect on vitrification of GV-porcine oocytes.

TL;DR: The effects of freezing procedures, osmolarity, trehalose, and sucrose on survival of bull sperm in whole milk and egg yolk-Tris, used worldwide, were studied and provide a basis for further investigating simple freezing systems that might be more effective in preserving bull sperm than those currently available.

TL;DR: The cryobiological preservation of mouse spermatozoa has presented difficulties in the form of poor motilities or irreproducibility, and the consequences of reducing the oxygen to <3% of atmospheric by the use of a bacterial membrane preparation, Oxyrase, are determined.

TL;DR: Data demonstrate that improvements to the concentration of cryoprotectant and addition procedures have critical effects on the developmental competence of oocytes vitrified in straws.

TL;DR: Elevated levels of enzymes and metabolites that cooperatively scavenge O2 and H2O2 were found, along with increased freezing tolerance during cold acclimation, supporting the hypothesis that free-radical scavenging systems may be important in the alleviation of freezing stress.

TL;DR: The results showed that ram spermatozoa undergo a dramatic loss of heterogeneity, viability, motility, and positive response to the HOS test during freezing and thawing, which was partially prevented by diluting samples in Fiser's extender containing egg yolk and glycerol.

TL;DR: The feasibility of using sperm cryopreservation with amphibian species is demonstrated and motility and fertilization rates were similar with Me2SO but diverged with glycerol as cryoprotectant.

TL;DR: It is demonstrated that porcine embryos can be successfully cryopreserved by rapid cooling in EG-PVP and by careful dilution of the CPA after warming.

TL;DR: It is suggested that the greater depression of the freezing point of water due to the complex formation and hence the attendant increase in the viscosity near the freezing Point is the reason for the sluggish crystallization in these solutions.

TL;DR: In this paper, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze glycoproteins (AFGP) from the blood of Antarctic notothenioid fish.

TL;DR: Results are consistent with the achieved fertility rates that demonstrated sublethal cryodamage in the function of the sperm membrane that was not detected by standard procedures.

TL;DR: It is indicated that periodic warming during cold storage increases survival by allowing a chilling intolerant stage to develop to a more tolerant stage and/or by eliminating accumulated toxic metabolites.

TL;DR: While the water permeability of the dechorionated zebrafish embryos at different developmental stages remained relatively stable, the permeability to the cryoprotectant methanol (Ps) appeared to decrease during embryo development.

TL;DR: It is concluded that there may be many mechanisms involved, but the most important factor is probably stresses produced by unphysiological cell-cell contacts occurring during the freeze-thaw cycle.

TL;DR: The results suggest that the membrane proteins of spermatozoa of the species of trout that were studied possess high motility and diffuse, with the formation of clusters, in very short periods of time (about 30 s).

TL;DR: It was concluded that human CD34+CD33- cells osmotically behave as ideal osmometers and this information coupled with cell water and cryoprotectant permeability coefficients as well as their activation energies will be used to design optimum conditions for cryopreservation of human hematopoietic progenitor cells.

TL;DR: The demonstrated standardized perfusion technique was achieved by a combination of special equipment and computer-aided monitoring and allows further experiments to improve understanding of ischemic and reperfusion injury.

TL;DR: The protection of the H+-ATPase by trehalose suggests that this carbohydrate might protect other membrane enzymes from inactivation during handling.

TL;DR: The crystallization of ice in aqueous solutions of glycerol and dimethyl sulfoxide (Me2SO) has been studied using a combined DSC-video microscope technique and the growth rates were found to be generally independent of time, particularly at lower temperatures.

TL;DR: Results indicate that low temperature causes an increased association of some proteins with the two chaperones, consistent with the hypothesis that normal biogenesis or the conformational stability of specific proteins may be unfavorably altered at low temperature in spinach and perhaps other plants.

TL;DR: The PCV and percentage of motile sperm suspended in EYT responded to osmotic changes similar to those reported for Tyrode's solution at both 25 and 5 degreesC, which may have application in improving bull sperm cryopreservation.

TL;DR: It is suggested that a loss of membrane integrity coupled with an inhibition of Complexes I and V and an involvement of Complex II-III in 24-h stored hepatic transplants accounts for mitochondrial respiratory dysfunction in hepatic transplantation injury.

TL;DR: The cryopreservation of isolated fish blastomeres is likely to provide a valid alternative to embryo cryop Reservation, but the results of which are still discouraging.