G. Gárdos

TL;DR: In inside-out red cell membrane vesicles active calcium transport and the formation of the enzyme-phosphate complex (EP) of the calcium pump were simultaneously investigated and the effects of a limited proteolytic digestion examined, suggesting a probable molecular arrangement for the functional parts of the red cell membranes calcium pump.

TL;DR: Results indicate the presence of the sarco-endoplasmic reticulum-type calcium transport ATPase 2b isoform and of a yet unidentified, 97-kDa calcium pump protein in human platelet membranes.

TL;DR: Sealed, inside-out human red cell membrane vesicles accomplish an ATP and Mg2+-dependent uphill calcium uptake with a reproducible maximum rate of 12-15 nmol/mg vesicle protein per min under physiological conditions, and the regulation of active calcium transport in human red cells is discussed.

TL;DR: Findings indicate the simultaneous presence of two different endoplasmic-reticulum Ca 2+ pumps in a variety of human cell types, and may explain the previously observed differences in the Ca(2+)-handling characteristics of different intracellular Ca2+ pools and cell types.

TL;DR: The presence of a calmodulin-binding regulatory subunit of the red cell calcium pump at the internal membrane surface is postulated and the system is converted to a “high calcium-affinity” state.