G
Gek Kee Sim
Researcher at Columbia University
Publications - 8
Citations - 3239
Gek Kee Sim is an academic researcher from Columbia University. The author has contributed to research in topics: Gene & Transformation (genetics). The author has an hindex of 7, co-authored 8 publications receiving 3223 citations. Previous affiliations of Gek Kee Sim include Harvard University.
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Journal ArticleDOI
The isolation of structural genes from libraries of eucaryotic DNA
Tom Maniatis,Ross C. Hardison,Elizabeth Lacy,Joyce Lauer,Catherine O'Connell,Diana Quon,Gek Kee Sim,Argiris Efstratiadis +7 more
TL;DR: A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.
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Transformation of mammalian cells with genes from procaryotes and eucaryotes
Michael Wigler,Raymond W. Sweet,Gek Kee Sim,Barbara Wold,A. Pellicer,Elizabeth Lacy,Tom Maniatis,Saul Silverstein,Richard Axel +8 more
TL;DR: This co-transformation system should allow the introduction and stable integration of virtually any defined gene into cultured cells and is likely to represent a subpopulation of competent cells which are likely to integrate other unlinked genes at frequencies higher than the general population.
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Altering genotype and phenotype by DNA-mediated gene transfer
Angel Pellicer,Diane M. Robins,Barbara Wold,Ray Sweet,James F. Jackson,Israel Lowy,James M. Roberts,Gek Kee Sim,Saul Silverstein,Richard Axel +9 more
TL;DR: Transformation provides an in vivo assay for the functional role of DNA sequence organization about specific genes and may provide a general approach to the analysis of complex heritable phenotypes by permitting the distinction between phenotypic changes without concomitant changes in DNA and functional genetic rearrangements.
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Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families
TL;DR: The characterized cDNA clones can now be used as probes for studying the evolution, chromosomal organization and regulated developmental expression of the chorion multigene families.