Graham J. Ray

TL;DR: By rationally designing single-stranded DNA donors of the optimal length complementary to the strand that is released first, this work increases the rate of HDR in human cells when using Cas9 or nickase variants to up to 60%.

TL;DR: A discovery platform that can identify stimulus-responsive enhancers for a target gene independent of stimulus exposure is described and reveals how non-coding variation associated with human immune dysfunction alters context-specific gene programs.

TL;DR: It is found that non-homologous single-stranded DNA greatly stimulates Cas9-mediated gene disruption in the absence of homology-directed repair, dramatically increasing the frequency of gene disruption.

TL;DR: In this paper, the authors provide methods and compositions for enhanced editing of genomic DNA, including linearized non-homologous DNA composition and a genome targeting composition (which includes a genome editing endonuclease, or a nucleic acid encoding the genome-e.g., a CRISPR/Cas guide RNA).

TL;DR: It is found that linear, non-homologous oligonucleotide DNA greatly stimulates Cas9-mediated gene disruption in the absence of homology-directed repair, dramatically increasing the frequency of gene disruption.