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William J. Greenleaf
Researcher at Stanford University
Publications - 241
Citations - 29961
William J. Greenleaf is an academic researcher from Stanford University. The author has contributed to research in topics: Chromatin & Gene. The author has an hindex of 60, co-authored 202 publications receiving 20226 citations. Previous affiliations of William J. Greenleaf include Harvard University & Weizmann Institute of Science.
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Journal ArticleDOI
Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position
TL;DR: The feasibility of analyzing an individual's epigenome on a timescale compatible with clinical decision-making is demonstrated and classes of DNA-binding factors that strictly avoided, could tolerate or tended to overlap with nucleosomes are discovered.
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ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide
TL;DR: This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin, which can be used to infer regions of increased accessibility, as well as to map regions of transcription‐factor binding and nucleosome position.
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Single-cell chromatin accessibility reveals principles of regulatory variation
Jason D. Buenrostro,Beijing Wu,Ulrike M. Litzenburger,David Ruff,Michael L. Gonzales,Michael Snyder,Howard Y. Chang,William J. Greenleaf +7 more
TL;DR: A robust method for mapping the accessible genome of individual cells by assay for transposase-accessible chromatin using sequencing (ATAC-seq) integrated into a programmable microfluidics platform is developed and single-cell analysis of DNA accessibility provides new insight into cellular variation of the ‘regulome’.
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An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
M. Ryan Corces,Alexandro E. Trevino,Emily G. Hamilton,Peyton Greenside,Nicholas A Sinnott-Armstrong,Sam Vesuna,Ansuman T. Satpathy,Adam J. Rubin,Kathleen S. Montine,Beijing Wu,Arwa Kathiria,Seung Woo Cho,Maxwell R. Mumbach,Ava C. Carter,Maya Kasowski,Lisa A. Orloff,Viviana I. Risca,Anshul Kundaje,Paul A. Khavari,Thomas J. Montine,William J. Greenleaf,Howard Y. Chang +21 more
TL;DR: The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures.
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Chromatin accessibility and the regulatory epigenome.
TL;DR: The goal is to illustrate how chromatin accessibility defines regulatory elements within the genome and how these epigenetic features are dynamically established to control gene expression.