Showing papers by "Guleray Agar published in 2016"
TL;DR: This study has evaluated the levels of antioxidants of human whole blood plasma in order to clarify the possible mechanisms that may contribute to the anti-genotoxic activity of the luteolin derivatives.
Abstract: In the present study, we aimed to investigate the genotoxic and anti-genotoxic potencies of three luteolin derivatives (luteolin-7-O-glucoside, luteolin-7-O-rutinoside and luteolin-7-O-glucuronide) by using human cells. In the micronucleus test, the human lymphocytes were exposed to aflatoxin B1, the luteolin derivatives and a mixture of the two for 72 h. Furthermore, we have evaluated the levels of antioxidants of human whole blood plasma in order to clarify the possible mechanisms that may contribute to the anti-genotoxic activity of the luteolin derivatives. According to the results obtained from the micronucleus test, the highest protection rates for luteolin-7-O-glucoside, luteolin-7-O-rutinoside and luteolin-7-O-glucuronide against aflatoxin B1 were 32.09, 35.55 and 37.50 %, respectively. Similarly, these three luteolin derivatives ameliorated the level of antioxidants altered from aflatoxin B1.
19 citations
TL;DR: The results of this experiment have clearly shown that CME has a significant antioxidative and antigenotoxic effect, which is thought to be due to the antigenot toxic activities of antioxidant enzymes.
Abstract: In this article, the genotoxic and antigenotoxic effects of methanol extract of of Cladonia foliacea (Huds.) Willd. (CME) were studied using WP2, Ames (TA1535 and TA1537), and sister chromatid exch...
15 citations
TL;DR: It can be suggested that HA applications removes the negative effects of Mn on retrotransposon polymorphism and GTS, when considering the research results.
14 citations
TL;DR: Results indicated that genomic template stability was significantly affected at the above fipronil concentration, and confirmed that the RAPD assays are useful in determining potential genotoxicity of fipronsil.
Abstract: This study was undertaken to appreciate genotoxic potential of fipronil herbicides in the roots of Vicia faba seedlings. Fipronil was clearly dose dependent on root growth inhibitors, total soluble protein, and random amplified polymorphic DNA (RAPD) assays. Increase in concentration of fipronil resulted in decreased amount of root length and increased level of protein. For the RAPD analyses, 15 RAPD primers were found to produce unique polymorphic band patterns and were subsequently used to produce a total of 110 bands of 45-1250 bp. Each primer generated 4-11 RAPD bands across 15 primers. The changes occurring in RAPD profiles following fipronil treatment included variation becoming evident as disappearance and/or appearance of DNA bands compared with the normal seedlings. These results indicated that genomic template stability was significantly affected at the above fipronil concentration. This study further confirmed that the RAPD assays are useful in determining potential genotoxicity of fipronil.
13 citations
TL;DR: The results indicate that RAPD and CRED-RA techniques can be used for detection of somaclonal variation in in vitro cultures, which is a fundamental step in plant genetic transformation.
Abstract: Genetic variations, and especially somaclonal variations, are undesirable in genetic transformation. In this study, random amplified polymorphic DNA (RAPD) and coupled restriction enzyme di...
11 citations
TL;DR: The essential oil of O. rotundifolium exhibits considerable antibacterial effect against pathogenic bacteria that cause significantly crop loss.
Abstract: In this study, the antibacterial effect of Origanum rotundifolium Boiss’s essential oil was evaluated against 20 plant pathogenic bacteria. The essential oil (EO) of O. rotundifolium was hydrodistillated from wild O. rotundifolium plants that grown in Eastern Anatolia region of Turkey. Then, 10 chemical compounds of EO were identified via gas chromatography/mass spectrometry (GC-MS). The main compounds were determined as thymol (40.86%-GC), carvacrol (43.62%), p-cymene (5.95%) and borneol (2.49%). According to the results of the study, the essential oil of O. rotundifolium exhibits considerable antibacterial effect against pathogenic bacteria that cause significantly crop loss.
11 citations
TL;DR: Results suggested that low intensity static magnetic field-induced DNA damage and methylation in wheat calli may trigger genomic instability and DNA methylation.
Abstract: Deoxyribonucleic acid (DNA) is always damaged by endogenous and exogenous factors. Magnetic field (MF) is one of these exogenous factors. When repair mechanisms are not sufficient, mainly because of imbalance in damage or mistakes in repair mechanisms, methylation of DNA results in polymorphism-related abnormalities. In this study, low intensity static magnetic field-induced DNA damage and methylation in wheat calli were investigated by using Random Amplified Polymorphic DNA and Coupled Restriction Enzyme Digestion-Random Amplification techniques. Calli were derived from mature embryos of wheat. Both 7- and 14-day-old wheat calli were exposed to 7 mT (millitesla) static MF for 24, 48, 72, 96, or 120 h of incubation period. The highest change in polymorphism rate was obtained in calli exposed to 7 mT MF for 120 h in both 7- and 14-day-old calli. Increase in MF duration caused DNA hypermethylation in both 7- and 14-day-old calli. Polymorphism and DNA methylation ratio were higher in 7-day-old calli. The highest methylation level with a value of 25.1% was found in 7-day-old calli exposed to MF for 120 h. Results suggested that low intensity static magnetic field may trigger genomic instability and DNA methylation. Bioelectromagnetics. 37:504–511, 2016. © 2016 Wiley Periodicals, Inc.
11 citations
TL;DR: RAPD and ISSR analysis are suggested to be useful for the differentiation of the Aethionema species growing in the wild in Eastern Anatolia, Turkey and may reveal differences in their origin.
9 citations
TL;DR: Thirty one pears genotypes from east blacksea region were evaluated for genetic relationships by using Randomly Amplified Polymorphic DNA (RAPD) and ISSR (Inter-Simple Sequence Repeats) markers from total 70 primer investigated, 22 could amplify clearly and consistently.
Abstract: Thirty one pears genotypes from east blacksea region were evaluated for genetic relationships by using Randomly Amplified Polymorphic DNA (RAPD) and ISSR (Inter-Simple Sequence Repeats) markers from total 70 RAPD and ISSR primer investigated, 22 could amplify clearly and consistently. Cluster analysis of the pears genotypes was performed based on data from polymorphic bands RAPD and ISSR by using Jaccard’s similarity coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. The 31 pear genotypes were classified into two major groups. Cluster A was divided into 2 subclusters: Gumushane pears and Trabzon pears. Cluster B consisted of Artvin pears. The similarity matrix values ranged between 0.105 and 0.968.
9 citations
TL;DR: It was observed that the levels of antioxidant enzymes were decreased dramatically and level of malondialdehyde (MDA) increased after treatment with AFB1 but the antioxidants enzymes were increased and MDA levels decreased in case of applying the lichen methanol extracts.
Abstract: The protective role of lichens are getting more important. In this study, the antigenotoxic and antimutagenic activity of the methanol extracts of four lichen species (Anaptychia ciliaris, Bryoria fuscescens, Parmotrema chinensa and Xanthoria candelaria) were investigated. Sister Chromatid Exchange (SCE) test were used for determining the genotoxic contamination and the results showed that four lichen extracts inhibited the mutagenic effects of AFB1. Three different concentrations used and it was determined that the most effective concentration is 20 μg/mL againts the mutagenic effects of AFB1. In addition, the antioxidant effects of A. ciliaris, B. fuscescens, P. chinensa and X. candelaria were determined by measuring the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH) and level of malondialdehyde (MDA) against the oxidative stress of AFB1 in human lymphocytes in vitro. It was observed that the levels of antioxidant enzymes were decreased dramatically and level of malondialdehyde (MDA) increased after treatment with AFB1 but the antioxidant enzymes were increased and MDA levels decreased in case of applying the lichen methanol extracts. We also determined that our lichen samples have strong antioxidative and antigenotoxic character and their antigenotoxic quality may be relation to the mechanisms of strong antioxidative property.
5 citations
TL;DR: The results of this experiment showed that MSA has strong antioxidative and antigenotoxic effects and this antigenotoxicity activities of MSA can be due to the antioxidant activities.
Abstract: In this research, the genotoxic and antigenotoxic effects of methanol extract of Sempervivum armenum (MSA) were studied using micronucleus (MN) test and sister chromatid exchange (SCE) test systems in cultured human peripheral blood cells. According to the SCE and MN tests results, MSA reduced the genotoxic effects of aflatoxin B1. In order to explain the reason for the antigenotoxic effects of MSA, antioxidants levels were determined. Cotreatments of 5, 10, 20 mg/mL concentrations of MSA with aflatoxin B1 decreased the frequencies of SCE, MN and the malondialdehyde level and increased the amount of superoxide dismutase, glutathione and glutathione peroxidase which were decreased by aflatoxin. The results of this experiment showed that MSA has strong antioxidative and antigenotoxic effects and this antigenotoxic activities of MSA can be due to the antioxidant activities.