Guy Hervé

TL;DR: Hydrolysis of β‐lactoglobulin with thermolysin and pepsin at pressures ranging between 0.1 and 350 MPa showed a significant increase of cleavage rates, indicating combined influence of pressure‐induced thermoly sin activation and partial unfolding of β-lactogobulin by compression at neutral pHs.

TL;DR: The pH dependence of the stimulation by ATP and adenylyl methylenediphosphonate indicates that the binding of these nucleotides requires the ionization of their last phosphate acidic group, providing additional evidence that homotropic and heterotropic interactions correspond to different molecular mechanisms.

TL;DR: 2-ThioU-aspartate transcarbamylase, a modified form of the enzyme in which the homotropic cooperative interactions between the catalytic sites are selectively abolished, shows the same heterogeneity in CTP binding sites as normal aspartates transcarb amylase.

TL;DR: A sequence in the central region of the protein and two short sequences in the C-terminal region are good candidates for the nuclear location signal involved in the targeting of the URA2 product.

TL;DR: A high pressure reactor for steady state studies of enzymes is described, which can be used for any enzymatic reaction; unlike previously described high pressure apparatus, it is not limited to the study of enzymes whose activity can be directly followed by spectrophotometry.