H
Hennie R. Hoogenboom
Researcher at Maastricht University
Publications - 85
Citations - 18195
Hennie R. Hoogenboom is an academic researcher from Maastricht University. The author has contributed to research in topics: Phage display & Antibody. The author has an hindex of 51, co-authored 85 publications receiving 17946 citations.
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Journal ArticleDOI
By-passing immunization: Human antibodies from V-gene libraries displayed on phage
James D. Marks,Hennie R. Hoogenboom,Timothy Peter Bonnert,John McCafferty,Andrew D. Griffiths,Greg Winter +5 more
TL;DR: The results suggest that a single large phage display library can be used to isolate human antibodies against any antigen, by-passing both hybridoma technology and immunization.
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Making antibody fragments using phage display libraries
TL;DR: Using a random combinatorial library of the rearranged heavy and kappa light chains from mice immune to the hapten 2-phenyloxazol-5-one (phOx), diverse libraries of antibody fragments are displayed on the surface of fd phage and elicited many more pairings with strong binding activities.
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Making Antibodies by Phage Display Technology
TL;DR: Human antibody fragments with many different binding specificities have been isolated from the same phage repertoire, including haptens, carbohydrates, secreted and cell surface proteins, viral coat proteins, and intracellular antigens from the lumen of the endoplasmic reticulum and the nucleus.
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Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains
Hennie R. Hoogenboom,Andrew D. Griffiths,Kevin Stuart Johnson,David John Chiswell,Peter J. Hudson,Greg Winter +5 more
TL;DR: Heterodimeric Fab fragments can be assembled on the surface of the phages by linking one chain to the phage coat protein, and secreting the other into the bacterial periplasm by introducing an amber mutation.
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By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro.
Hennie R. Hoogenboom,Greg Winter +1 more
TL;DR: In this article, a repertoire of human VH genes from 49 human germline VH gene segments was rearranged in vitro to create a synthetic third complementarity determining region (CDR) of five or eight residues.