Hervé Pagès

TL;DR: The first genome-scale map of murine NADs in mouse embryonic fibroblasts (MEFs) is determined via deep sequencing of chromatin associated with purified nucleoli via Bioconductor package called NADfinder and it is demonstrated that it identifies NADs more accurately than other peak-calling tools, owing to its critical feature of chromosome-level local baseline correction.

TL;DR: An overview of single-cell RNA sequencing analysis for prospective users and contributors is presented, highlighting the contributions towards this effort made by Bioconductor.

TL;DR: The GUIDEseq package enables analysis of GUIDE-data from various nuclease platforms for any species with a defined genomic sequence and annotates potential off-target sites that overlap with genes based on genome annotation information, as these may be the most important off- target sites for further characterization.

TL;DR: The most popularnotation resources in the Bioconductor project are described and some high level examples on how to use them are given.

TL;DR: A C++ interface named beachmat is described, which enables agnostic data access from various matrix representations and allows package developers to write efficient C++ code that is interoperable with dense, sparse and file-backed matrices, amongst others.