S
Scot A. Wolfe
Researcher at University of Massachusetts Medical School
Publications - 151
Citations - 11964
Scot A. Wolfe is an academic researcher from University of Massachusetts Medical School. The author has contributed to research in topics: Genome editing & Gene. The author has an hindex of 49, co-authored 130 publications receiving 10004 citations. Previous affiliations of Scot A. Wolfe include Washington University in St. Louis & University of Illinois at Urbana–Champaign.
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Journal ArticleDOI
DNA recognition by Cys2His2 zinc finger proteins.
TL;DR: This review analyzes DNA recognition by Cys2His2 zinc fingers and summarizes progress in generating proteins with novel specificities from fingers selected by phage display.
Journal ArticleDOI
A Novel miRNA Processing Pathway Independent of Dicer Requires Argonaute2 Catalytic Activity
Daniel Cifuentes,Huiling Xue,David W. Taylor,Heather Patnode,Yuichiro Mishima,Yuichiro Mishima,Sihem Cheloufi,Sihem Cheloufi,E. Ma,Shrikant Mane,Gregory J. Hannon,Nathan D. Lawson,Scot A. Wolfe,Antonio J. Giraldez +13 more
TL;DR: It is proposed that Ago2-mediated cleavage of pre-miRNAs, followed by uridylation and trimming, generates functional miRNAs independently of Dicer, which suggests that other mi RNAs might also be processed in this way.
Journal ArticleDOI
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
TL;DR: The use of engineered ZFNs to introduce heritable mutations into a genome obviates the need for embryonic stem cell lines and should be applicable to most animal species for which early-stage embryos are easily accessible.
Journal ArticleDOI
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo
Hao Yin,Chun-Qing Song,Joseph R. Dorkin,Lihua Julie Zhu,Yingxiang Li,Qiongqiong Wu,Angela I. Park,Junghoon Yang,Sneha Suresh,Aizhan Bizhanova,Ankit Gupta,Mehmet Fatih Bolukbasi,Stephen Walsh,Roman L. Bogorad,Guangping Gao,Zhiping Weng,Yizhou Dong,Victor Koteliansky,Victor Koteliansky,Scot A. Wolfe,Robert Langer,Wen Xue,Daniel G. Anderson +22 more
TL;DR: The delivery strategy is applied to a mouse model of human hereditary tyrosinemia and it is shown that the treatment generated fumarylacetoacetate hydrolase (Fah)-positive hepatocytes by correcting the causative Fah-splicing mutation and rescued disease symptoms such as weight loss and liver damage.
Journal ArticleDOI
Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish
Fatma O. Kok,Masahiro Shin,Chih-Wen Ni,Ankit Gupta,Ann S. Grosse,Andreas van Impel,Bettina C. Kirchmaier,Josi Peterson-Maduro,George Kourkoulis,Ira Male,Dana F. DeSantis,Sarah Sheppard-Tindell,Lwaki Ebarasi,Lwaki Ebarasi,Christer Betsholtz,Christer Betsholtz,Stefan Schulte-Merker,Scot A. Wolfe,Nathan D. Lawson +18 more
TL;DR: It is suggested that mutant phenotypes become the standard metric to define gene function in zebrafish, after which Morpholinos that recapitulate respective phenotypes could be reliably applied for ancillary analyses.