Hildur V. Colot

TL;DR: This study describes a method for rapidly creating knockout mutants in which it makes use of yeast recombinational cloning, Neurospora mutant strains deficient in nonhomologous end-joining DNA repair, custom-written software tools, and robotics.

TL;DR: The strong mutant phenotype, association of the protein with NPCs and genetic interaction with factors involved inRNA export provide strong evidence that Dbp5p/Rat8p plays a direct role in RNA export.

TL;DR: CDNA libraries generated in Project 4 document the overall complexity of expressed sequences in Neurospora, including alternative splicing alternative promoters and antisense transcripts, and drive the assembly of an SNP map presently populated by nearly 300 markers that will greatly accelerate the positional cloning of genes.

TL;DR: It is reported that Dbp5p and Rat7p interact through their Nterminal domains, and overexpression of DBP5p prevents nuclear accumulation of mRNA in xpo1‐1 cells, but does not restore growth, suggesting that the RNA export defect of xPO1‐ 1 cells may be indirect.

TL;DR: Evolutionary comparisons with the Sordariaceae reveal conservation of 5' UTR sequences, as well as significant conservation of the alternative splicing events, supporting their relevance to proper regulation of clock function.